Surfactant And Polymeric Micelle-based Fluorescent Nanoprobes For CO Detection

The micelle structure assembled from amphiphilic molecules such as surfactants has a hydrophobic core and a hydrophilic shell,which has attracted special attention as a new type of nano-drug delivery system.In the field of molecular probes,micellar systems are used as carriers to improve the water solubility and sensitivity of probes.In this thesis,on the basis of the fact that CO molecules have higher solubility in the hydrophobic region of micelles,several CO molecular probes are synthesized,including small molecule probes,metal probes,and fluorescence resonance energy transfer(FRET)probes.They are loaded into surfactant micelles and polymer micelles for CO detection and analysis.Chapter 1: The concept of surfactants is briefly introduced,and the forming principle of micellar system and the synthesis and application of polymer micelles are comprehensively summarized.Introduction to the application progress of CO fluorescent probes and probe molecules in micellar systems.Chapter 2: Construction of a metal-free small molecule probe NIR-CO by using CO to reduce the nitro group to the amino moiety.With cationic surfactant cetyltrimethylammonium bromide(CTAB),the probe can detect CO in an aqueous solution.Through the characterization of ultraviolet-visible spectroscopy and fluorescence spectroscopy,different concentrations of CORM-2 were analyzed and detected.With the concentration of CORM-2,the fluorescence intensity had a good linear relationship with the detection limit of81 n M.The colorimetric characteristics of the probe have obvious advantages in field detection and "naked-eye" observation,this method provides a simple method for CO detection without the aid of equipment for color changes.Chapter 3: Construction of a a metal palladium complex probe NR-Pd A based on Nile Red to specifically respond to CO.By trapping the probe inside the hydrophobic surface cross-linked micelle(SCM),the probe is given good water solubility and high sensitivity.In the aqueous micellar system,the fluorescence intensity of the probe can reach more than 5 times the initial fluorescence within 30 minutes,which effectively improves the environmental limitations of the Nile Red dye probe.By comparing the micellar system of alkane chain and fluorocarbon chain,an easy-to-apply molecular visual detection system was constructed to visually detect CO gas.Chapter 4: Construction of Nano-CO,a probe that can specifically detect CO.Based on fluorescence resonance energy transfer(FRET)system,synthesize rhodium polymer Rh(II)and nitrobenzoxadiazole(NBD)molecules.The probe can be simply synthesized with good biocompatibility,stability and water solubility under the effect of polymer cross-linked micelles.Unexpectedly,with the increase of the time of adding CO,the lower the fluorescence intensity of the fluorescent probe,which is preliminarily speculated due to the influence of exciton effect in the polymer micelle system.Further exploration of its mechanism may provide new ideas for the application of probe molecules in micellar systems.