MIL-88（NH₂）-Fe@CNTs Based Colorimetric Method For Hydrogen Peroxide Detection

H₂O₂ is a green fine chemical product,which is widely used in chemical production and other fields.High concentrations of H₂O₂ entering the human body may produce excessive hydroxyl free radicals,which will seriously damage human health.Therefore,the detection of H₂O₂ is of great significance.Among the many detection methods of H₂O₂,the colorimetric method is convenient,fast and visual.With the development of nanotechnology,the use of nanozymes to develop colorimetric sensing detection methods has attracted widespread attention.In this thesis,a one-step hydrothermal method was used to synthesize CNTs-modified MIL-88（NH₂）-Fe.Using the peroxidase-like activity of MIL-88（NH₂）-Fe@CNTs,the effect of DNA on the MIL-88（NH₂）-Fe@CNTs was studied.Build a colorimetric sensor platform for H₂O₂,the specific content is as follows:（1）MIL-88（NH₂）-Fe@CNTs was prepared by hydrothermal synthesis,the peroxidase-like activity of MIL-88（NH₂）-Fe@CNTs was investigated,and the ability of DNA to regulate the peroxidase-like activity of the composite material MIL-88（NH₂）-Fe@CNTs was studied.Through the comparison of different reaction systems,it is found that the peroxidase-like activity of MIL-88（NH₂）-Fe@CNTs is higher than that of MIL-88（NH₂）-Fe.DNA can enhance the peroxidase-like activity of MIL-88（NH₂）-Fe@CNTs.Different base sequences,base concentrations,and base lengths reflect different regulatory abilities of peroxidase-like activity of MIL-88（NH₂）-Fe@CNTs,which enables MIL-88（NH₂）-Fe@CNTs to have a wider range of applications in the field of sensing.（2）Using the peroxidase-like activity of MIL-88（NH₂）-Fe@CNTs,a colorimetric sensing platform for H₂O₂ was constructed.Under optimized conditions,the linear detection range for H₂O₂ was 0.01-5 m M,and the detection limit was 10.4μM（3δ/s）.In order to study the effect of sensing method on the detection of H₂O₂ in actual samples,we took tap water and lake water as water samples,and adopted the method of adding standard recovery to detect H₂O₂ in the water samples.The recovery rate is between 90%-102.5%.The standard deviation is less than3.3%.In addition,we explored the practicality and commercialization of the sensing platform,and used this method to design and prepare H₂O₂ test papers.