The Preparation And Application Research Of CuO Nanocomposite Based On Electrospinning Technology

With the rapid development of nanotechnology,nanomaterials can improve the sensitivity and selectivity of sensors.CuO nanocomposites have better performance in electrocatalytic activity than the metal materials.Therefore,nano-CuO,nano-CuO@Ag₂O and nano-CuO@ZnO are synthesized by electrospinning technology and high temperature calcination technology.Three electrochemiluminescence sensors are constructed based on CuO nanocomposites.CuO nanocomposites are combined with ECL sensing technology and layer-by-layer self-assembly technology.The content of the trace drugs is detected.The main research work is as follows:1.Synthesis and characterization of CuO nanocomposites.CuO nanocomposites were prepared by high temperature calcination technology and electrospinning technology,and the conditions of synthesis were studied.During the synthesis of nano-CuO,1:1 ethanol aqueous was the solvent,and PVP/CuSO₄ was the solute.It was characterized by XRD and SEM.During the synthesis of nano-CuO@Ag₂O,DMF was the solvent,and PVP/PMMA/CuSO₄/AgNO₃was the solute.It was characterized by XRD and SEM.During the synthesis of nano-CuO@ZnO,DMF was the solvent,and PVP/PMMA/Cu（Ac）₂·H₂O/（Ac）₂Zn was the solute.Nano-CuO,nano-CuO@Ag₂O and nano-CuO@ZnO have excellent performance.They were used for follow-up application.2.Determination of Clenbuterol hydrochloride（CLB）by Au NPs@CuO/GCE ECL sensor.Au NPs solution was mixed with 1 mg·m L^-1 nano-CuO solution in 1:1 ratio.Au NPs@CuO/GCE ECL sensor was constructed by drop-coating.Due to the synergy of nano-CuO and Au NPs,and luminol could be catalyzed.At the same time,the intensity and stability of Luminol-O₂system were improved by Au NPs@CuO.So a new method for determination of CLB was established.The logarithm of CLB concentration had a good linear relationship with the relative ECL intensity in the range of 2.0×10^-10～2.0×10^-5mol·L^-1.The detection limit（S/N=3）was 6.7×10^-11 mol·L^-1.The sensor had excellent sensitivity,stability,selectivity and reproducibility CLB in pig samples had been successfully determined.3.Determination of Cef by CuO@Ag₂O/Co-HB/GCE ECL sensor.CuO@Ag₂O-Co-HB/GCE ECL sensor was prepared by layer-by-layer self-assembly technology.The large specific surface area of nano-CuO@Ag₂O could increase the carrying capacity of Co-HB,and Co-HB could amplify the signal in Ru（bpy）₃²⁺-Tpr A system.So a new ECL sensor of CuO@Ag₂O-Co-HB/GCE was constructed.According to the results,Cef had a significant quenching effect on ECL signal.The detection limit（S/N=3）was 7.47×10^-11 mol·L^-1.A new method for the determination of Cef was established,and it had great advantages in stability and specificity.4.Determination of L-DOPA by Nafion/CuO@ZnO/DNA/GCE ECL sensor.Salmon DNA used as the first layer,nano-CuO@ZnO could be adsorbed by DNA.Nano-CuO@ZnO was the second layer.It had the large surface area,and luminol could be loaded on nano-CuO@ZnO.Then Nafion was protective film,and the stability of the Nafion/CuO@ZnO/DNA/GCE sensor could be improved.The logarithm of CLB concentration had a good linear relationship with the relative ECL intensity in the range of 2.0×10^-10～2.0×10^-5mol·L^-1.The detection limit（S/N=3）was 6.7×10^-11mol·L^-1.The Nafion/CuO@ZnO/DNA/GCE ECL sensor had excellent stability and specificity.The content of L-DOPA was successfully detected in actual samples.