Study On The Analytical Method Of Flavonoids In Sophora Japonica By Three-dimensional Fluorescence And Chemometrics

Different countries have different management policies and safety standards for traditional medicine,and issues such as safety and effectiveness have become the main factors influencing the entry of traditional Chinese medicine into the international market.Spectroscopy is a relatively sensitive and fast detection method.Among them,three-dimensional fluorescence spectroscopy has the characteristics of comprehensive spectral information,high sensitivity,and intuitive images.This subject uses three-dimensional fluorescence spectroscopy combined with related chemometric analysis methods to analyze and study the flavonoids in Sophora japonica.The main research contents are as follows:(1)Study on the three-dimensional fluorescence and oblique projection analysis method of flavonoids in Sophora japonica.The fluorescence and absorption spectra of flavonoids in Sophora japonica were studied,and a new method was established to determine the content of flavonoids in extracts of Sophora japonica with three-dimensional fluorescence data and oblique projection algorithm.Flavonoids can detect the fluorescence characteristic peaks by a three-dimensional fluorescence spectrometer,and process the obtained fluorescence data with oblique projection algorithm to achieve the purpose of quantification.The experimental results show that the three-dimensional fluorescence combined with the oblique projection algorithm can better calculate the content of flavonoids in Sophora japonica,the correlation coefficient r=0.9966,the standard recovery rate is between92.69%and 100.96%,compared with liquid chromatography The relative error of the detection result is less than 5.79%,and the result is better than the parallel factor algorithm.(2)Quality analysis of Sophora japonica from different origins and construction of fingerprints.The commercially available Sophora japonica from different producing areas was extracted by alkaline extraction and acid precipitation.The content and purity of rutin in the extract were determined by liquid chromatography,so as to analyze the quality of Sophora japonica.Perform liquid chromatography and three-dimensional fluorescence spectroscopy on Sophora japonica extracts from different producing areas.Establish fingerprints of different types of Sophora japonica extract,and compare the differences between liquid chromatographic fingerprints and three-dimensional fluorescence fingerprints.The results showed that the rutin content and purity of Guangxi Golden Sophora extract is the highest.Based on the crude extract of rutin,the yield of rutin in Guangxi Golden Sophora extract was as high as 41.30%,and the purity reached 82.32%.After roasting,the content of Sophora japonica changed and the tannins increased.This result was consistent with the analysis result of liquid chromatographic fingerprint,indicating that the three-dimensional fluorescence fingerprint could be used for the identification of actual Sophora japonica Chinese medicinal materials.(3)Rapid analysis of the leaching process of flavonoids in Sophora japonica.Collect the three-dimensional fluorescence spectra of the solution of Sophora japonica in the process of soaking in different solvents,temperatures,times and solvents,mainly investigate the three-dimensional fluorescence characteristics of the leaching solution.Combined with the mathematical model formula of Chinese herbal medicine extraction,the kinetics of the extraction of flavonoids in Sophora japonica was studied.The results show that when water is used as the extraction solvent,the optimal conditions for extraction of flavonoids in Sophora japonica are the extraction temperature of 363K,the time is not less than 110min,and the solvent volume is not more than 100;when methanol is used as the solvent,the extraction The better conditions are temperature 333K,time no less than 80min,and solvent multiples no more than 100;in the study of extraction kinetics,ln C has a linear relationship with lnt,ln(M-R),1/T×10~3,respectively.It conforms to the kinetic model of Chinese herbal medicine extraction.