Study On Sugar Recognition And Gelation Based On Two-component Boric Acid Sensing System

Sugar is an important nutrient and energy supply substance,and it also participates in an astonishing number of biochemical processes in the organism.Excessive intake of glucose is directly related to type 2 diabetes;excessive intake of fructose may also increase the probability of cardiovascular disease and gout.Based on the principle that boric acid can reversibly form chemical bonds with glycols,boric acid sugar sensors are increasingly entering the center of the sugar recognition research arena.The twocomponent boric acid system innovatively separates the fluorophore part from the boric acid acceptor,allowing the boric acid part to act as a sugar recognition acceptor and a fluorescence quencher.Pyridine boronic acid and the fluorophore 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt(HPTS)constitute a two-component boric acid fluorescence sensor system,which is used in various sugar recognition studies and fructose detection in three fruits.Pyridine boronic acid 5 quenches HPTS.After glucose is added,the fluorescence of the quenched system recovers and has a linear relationship with the glucose concentration,indicating that the system is suitable for glucose sensing.A similar sugar binding experiment was performed on fructose,and the fructose concentration showed a linear relationship with the fluorescence recovery.Compared with the national standard method,the relative error of the fruit samples tests was within 5%.The above sugar sensing process was visualized through a portable UV lamp.The color changes when different ratios of 5 was added to quench HPTS,and the color change of fluorescence emission under the stimulation of different sugar concentration is also significant.Different types of sugars also display different colors during the sensing process.Among them,the color of fructose sensing is the darkest,which is dark yellowgreen.The double bond pyridine boronic acid 9 and acrylamide and HPTS constitute a hydrogel sensing system.After HPTS was quenched by 9 in the system,the best quencher/fluorophore ratio was obtained by fitting the data of glucose binding.The fluorescence recovery of the gel sensor system changes linearly with the glucose concentration,indicating that it has the potential to be used as a basic material for blood glucose concentration monitoring.