Research On Iron-based Nanomaterials To Promote The Generation Of Reactive Oxygen Species And Its Anti-Tumor Effect

Reactive oxygen species（ROS）play an important role in the cell life cycle（such as proliferation）,but when the ROS level exceeds the threshold required for normal physiological activity of tumor cells,DNA and protein will be inactivated,which further leads to cell death.Therefore,it is a feasible strategy to treat cancer cells by exogenous intervention to break the balance of intracellular ROS.In order to greatly improve the oxidation efficiency of tumor cells,Fenton reaction or Fenton-like reaction is needed,and H₂O₂ is converted into·OH in the presence of Fenton reagents such as iron(Fe²⁺).Therefore,by exogenously increasing the content of H₂O₂ in the cell,or exogenously increasing the catalyst of H₂O₂ such as iron ions and by reducing the content of antioxidants in cancer cells.Therefore,iron-based nanomaterials are the most widely used nanopharmaceutical materials for the treatment of tumors by Fenton reaction.Based on the above research,this article proceeds from the following three aspects:1.Preparation and characterization based on iron-copper nano-alloys and preliminary determination of catalytic performance.In this chapter,a solvothermal method was used to synthesize iron-copper nano-alloys（FC）with ultra-small particle size,and the surface was modified with BSA to form iron-copper nano-alloys with stable morphology and good biocompatibility FCB).At the same time,the results of extracellular experiments show that FCB has the ability to catalyze Fenton reaction and reduce GSH under acidic conditions.2.Research on anti-tumor activity in vivo and in vitro based on iron-copper nano alloy.On the basis of the previous chapter,we first investigated the catalytic performance of FCB in cells and the treatment of cancer cells,and then explored the anti-tumor effect of FCB in mice by constructing animal models.The experimental results show that:the content of·OH in MCF-7 cells after FCB incubation increases,the content of GSH decreases,and the amount of H₂O₂ also increases by a certain amount.At the same time,when MCF-7 cells were incubated with FCB at a concentration of 100μg/m L for 24 h,the survival rate of MCF-7 cells was only 31.6%.In animal model experiments,FCB also showed a significant tumor suppressing effect,and tissue staining also showed that other organs of mice were not damaged.The results show that FCB provides a good example and foundation for the application of alloy nanomaterials in the field of tumor therapy.3.Synthesis of PEG-PLGA polymer micelle based on salicylic acid and its application in tumor cells.In this chapter,by using PEG-PLGA as a carrier,self-assembling and loading salicylic acid and ferrocenecarboxylic acid by the emulsification and evaporation method,a uniform and stable nanomicelle with good biocompatibility is formed.These two drugs are designed to increase the levels of iron ions and H₂O₂ in the cells to trigger the Fenton reaction and increase ROS.The MTT experiment showed that this nanomicelle had a lethal rate of 77.2%to MCF-7 cells（24h）at a concentration of 80μg/m L.The above experimental results showed that the nanomicelle had a good tumor treatment effect.