Construction And Application Of Nano-Delivery System For Aerosol Inhalation Of Lung Through The Mucus Barrier

In recent years,with environmental problems intensifying,respiratory related diseases are common,among which pneumonia is harmful to the population.There are many ways to treat pneumonia.Aerosol inhalation has significant advantages over other routes:high drug concentration and low systemic toxicity.Due to the existence of mucus barrier in organism,the drugs will be blocked and cleared by the mechanism of size filtration and interaction filtration during conventional atomization administration,resulting in low bioavailability.Compared with the conventional drug delivery system,the advantages of nano-delivery system are that it can better penetrate the mucus barrier through surface modification,design size,targeting and so on,and increase stability,biological activity,reduce side effects and so on.Therefore,the interaction between nano-delivery system and pulmonary biological barrier is of great significance for atomization drug therapy.Based on the scientific problem of breaking through mucus barrier,two kinds of nano-delivery systems for pulmonary atomization inhalation were constructed by using nano-drug delivery system technology.Two kinds of drugs commonly used in the treatment of pneumonia,one is anti-inflammatory macromolecular protein drug(catalase),and the other is anti-infection small molecular antibiotic drug(polymyxin B),constructing the nano-delivery system for these two drugs with great difference in nature.According to the characteristics of the two drugs,the corresponding models of acute lung injury and bacterial pneumonia were established,and the atomization administration of mice was realized by using the technique of quantitative atomization syringe of pulmonary liquid on the model.The efficacy and distribution of nanopharmaceuticals in living mice were evaluated.The results showed that 2-methacryloyloxyethylphosphatidylcholine(MPC)nanomaterials and hyaluronic acid(HA)nanomaterials were used as carriers of drug delivery system.The inactivated catalase(CAT)and polymyxin B(PMB)with high toxicity can pass through the mucus barrier smoothly.