Near-infrared Fluorescent Nanoprobe Based On MOF Material And Its Application In Cancer Diagnosis And Treatment

Cancer,because of its high morbidity and mortality,brings huge pain and burden to many families and the whole society.However,clinical studies have shown that if cancer can be detected early and then matched with appropriate treatments,the vast majority of patients can be cured and there is no risk of recurrence.Adenosine triphosphate(ATP)is a vital multifunctional biomolecule,mainly located in mitochondria,widely involved in many cellular processes.According to reports,the concentration of ATP in cancer tissues is abnormally higher than that in normal tissues,so ATP can be used as a cancer biomarker.Zeolitic imidazole frameworks(ZIFs),as a new type of crystalline porous composite material,have good modifiability,designability and good biocompatibility.However,fluorescent nanoprobes based on ZIFs are also slowly developed.Therefore,if an ATP-responsive fluorescent nanoprobe can be constructed to deliver drugs by a controlled and precise on-demand manner,it can not only perform fluorescence imaging of tumor mice for diagnosis,but also overcome the obstacles of the single drug in the treatment of cancer.And it greatly improves the effect of cancer treatment.Therefore,we hope to achieve three types of methods are used to cancer diagnosis and controlled release of drugs.1.We first designed and synthesized an ATP-responsive near-infrared fluorescent nanoprobe(Rh I-DOX@ZIF-90)for cancer diagnosis and chemotherapy.The nanoprobe Rh I-DOX@ZIF-90 has high sensitivity and selectivity to ATP.Rh IDOX@ZIF-90 can target mitochondria,which provides a basis for improving drug delivery efficiency through mitochondrial administration.Therefore,the nanoprobes will preferentially concentrate on the tumor site,and use the near-infrared fluorescence signal by Rh I to perform fluorescence imaging of the tumor in tumor mice.At the same time,chemotherapeutic drugs(DOX)can also be controlled and accurately released at the tumor site to achieve highly effective anti-tumor effects.2.We designed and synthesized an ATP-responsive near-infrared fluorescent nanoprobe(Rh I-GOD@ZIF-90)for cancer diagnosis and starvation treatment.First of all,the probe has good selectivity to high concentrations of ATP in the tumor microenvironment.Therefore,the greatest degree of accumulation in tumor cells,and realize fluorescence imaging of tumor cells by releasing Rh I.Secondly,due to the selectivity of nanoprobe to tumor cells,it improves the effectiveness and bioavailability of GOD in inhibiting the growth of tumor cells.3.We designed and constructed an ATP-responsive near-infrared fluorescent nanoprobe(Rh I-GOD-DOX@ZIF-90)for cancer diagnosis and the collaborative treatment.First of all,the nanoprobe still shows high sensitivity and selectivity to ATP,and can be selectively enriched in tumor tissues.The Rh I released from the nanoprobe can be used to detect tumors in tumor mice.In order to achieve a diagnosis of cancer.More importantly,due to the synergistic effect of chemotherapy and starvation treatment,the nanoprobe exhibits a more excellent effect of inhibiting tumor growth.