Preparation Of Chemiluminescent Probe Of Biomimetic Antibody On ZnO Particle Surface And Its Detection For Mg

Malachite green is a toxic triphenylmethane chemical.It is not only a dye,but also a drug that kills fungi,bacteria and parasites.Long-term excessive use can cause cancer,and the addition of malachite green is prohibited by the state in the field of pollution-free aquaculture.However,MG is still illegally used in aquaculture.MG is a major chemical dye,and its production cannot be prohibited.Therefore,it is of great significance to strictly monitor the concentration of MG in aquatic products.Although it is called malachite green,it does not contain malachite,but the colors of the two are similar.Malachite and malachite green are two completely different substances.In this paper,the functionalized ZnO QDs were synthesized by sol-gel method.The fluorescence probe detection technology and molecular imprinting technology were combined to detect malachite green residues.Fluorescence detection of low concentration of malachite green ethanol solution in laboratory provides a scientific basis for the detection of malachite green and its metabolites in actual fish and water samples.Chapter 2,mainly introduces the luminescence mechanism,preparation method,development trend and application of ZnO quantum dots.Chapter 3,the preparation of zinc oxide quantum dots and the preparation and characterization of zinc oxide quantum dots modified by cysteine.The influence of cysteine concentration on the fluorescence properties of zinc oxide quantum dots is discussed.High resolution electron lens HRTEM analysis shows that the particle size of the product is uniform,about 5 nm.The as-prepared ZnO QDs were further characterized by XRD,FT-IR,UV-vis,FL and EDS.The results showed that there were almost no other peaks in the XRD spectra of the cysteine-modified ZnO QDs,indicating that the prepared ZnO QDs were pure phase.The results of infrared spectroscopy showed that the surface of cysteine modified zinc oxide quantum dots was rich in amino,indicating that cysteine was successfully modified on the surface of zinc oxide quantum dots.UV spectra showed that cysteine modified zinc oxide quantum dots were nanometer materials.The fluorescence spectra showed that the cysteine modified zinc oxide quantum dots emitted blue fluorescence,and the fluorescence was strong.EDS analysis showed that the surface of ZnO quantum dots was rich in hydroxyl,indicating that the modification of ZnO quantum dots by cysteine was successful,and the obtained ZnO quantum dots were stable in aqueous solution,and the fluorescence energy was strong.Chapter 4,the preparation of zinc oxide quantum dots and the preparation and characterization of（3-aminopropy）triethoxysilane modified zinc oxide quantum dots,the effect of APTS concentration on the fluorescence properties of ZnO QDs was also investigated.High resolution electron lens HRTEM analysis shows that the particle size of the product is uniform,about 5 nm.The ZnO QDs were further characterized by XRD,FT-IR,UV-vis,FL and EDS.The results showed that there were almost no other peaks in the XRD spectra of the cysteine-modified ZnO QDs,indicating that the prepared ZnO QDs were pure phase.The results of infrared spectroscopy showed that the surface of APTS modified ZnO quantum dots was rich in amino groups,indicating that the surface of ZnO quantum dots was successfully modified.UV spectrum results showed that APTS-modified ZnO QDs were nanoscale materials;the results of fluorescence spectra show that APTS modified ZnO quantum dots emit yellow fluorescence,and the fluorescence is strong.EDS analysis showed that the surface of ZnO QDs was rich in hydroxyl,indicating that APTS was successfully modified ZnO QDs,and the obtained ZnO QDs were stable in aqueous solution,and the fluorescence energy was strong.Then,SiO₂@ZnO@NH₂@MIP fluorescent probe was prepared with MG as template molecule.The thermodynamic and kinetic experiments of imprinted and non-imprinted materials were carried out.The results showed that when the ratio of SiO₂and NH₂-ZnO QDs was 1:2,the maximum saturated adsorption capacity of malachite green was 156.89 mg·g^-1,which was much higher than 47.38 mg·g^-1 of non-imprinted materials,indicating that SiO₂@ZnO@NH₂@MIP fluorescent probe had higher recognition performance than SiO₂@ZnO@NH₂@NIP fluorescent probe.In this paper,according to the preparation of ZnO QDs and the existing conditions,two kinds of surface modifiers were selected to modify ZnO QDs.The blue ZnO quantum dots stable water dispersion and strong yellow light emitting ZnO quantum dots stable water dispersion were synthesized by a simple sol-gel method.Finally,the preparation scheme of ZnO@NH₂ QDs with uniform particle size and strong fluorescence was selected to continue the next experiment.Combining molecular imprinting technology with fluorescence analysis technology,a fluorescent probe was invented for the detection of malachite green residues.The residual high-risk substances in the environment were detected by fluorescence resonance energy transfer principle.Based on the original intention of environmental protection,no secondary pollution in the detection process and simple detection operation,ZnO QDs were introduced into the internal of molecularly imprinted bionic antibody to prepare SiO₂@ZnO@NH₂ imprinted shell.The adsorption properties of malachite green were compared by the kinetic and thermodynamic experiments.High resolution transmission electron microscopy,scanning electron microscopy,infrared spectroscopy,ultraviolet spectroscopyand fluorescence spectroscopy were used to observe the morphology and structure of the SiO₂@ZnO@NH₂ shell.The results showed that when ZnO@NH₂QDs and SiO₂were 1:2,the SiO₂@ZnO@NH₂ shell had high adsorption performance and good heart-shell structure characteristics.Finally,the SiO₂@ZnO@NH₂bionic antibody had excellent single recognition ability for malachite green.