High-resolution Fluorescent Imaging Of Tumor Cells Of Amphiphilic Disulfide Bond Dicyanine Dye Nanoparticles

Fluorescence imaging technology is widely used in pathological detection and disease diagnosis.The cyanine-based fluorescent dye as a near-infrared fluorescent probe is different from the traditional near-infrared fluorescent probe.Under normal conditions,it has weak fluorescence and can be activated by GSH to produce strong fluorescence to improve the fluorescence imaging performance.However,cyanine dyes have low targeting properties and can be rapidly metabolized and eliminated by the liver and kidneys,which is not conducive to disease detection.In response to this problem,two amphiphilic disulfide bond biscyanine dyes（SO₃Cy7-ss-Cy7）and（SO₃Cy7-ss-Cy7.5）were designed and synthesized.The structure itself was amphiphilic and self-assembled in aqueous solution.Nanoparticles.Due to aggregation quenching,it does not emit fluorescence or only weak fluorescence.When it reacts with GSH,the disulfide bond breaks,resulting in decreased aggregation and strong fluorescence.The main research work is:designing two amphiphilic disulfide bond dicyanine dyes and studying their synthesis methods,using MODLI-TOF-MS,1H NMR,13C NMR,etc.to determine the structure of the target probe,and to self-assemble The morphology,particle size,etc.of nanoparticles are analyzed;UV-visible spectroscopy and fluorescence spectrophotometer are used to study the spectroscopic properties;the probes are tested for anti-interference ability,kinetic response,pH stability,cytotoxicity and Its live cell imaging,animal imaging,etc.are studied.The results show that both probes have good specificity,stability and sensitivity.The MTS method was used to determine the toxicity of two amphiphilic disulfide bond double cyanine fluorescent probes to He La cells.The two probes were used for live cell imaging using a confocal microscope and tumor-bearing nude mice were imaged in vivo.Cell-level test results show that the probe is almost non-toxic,live cell fluorescence imaging and in vivo imaging of tumor-bearing nude mice are clear.The probe has good tumor cell targeting and provides a new method for fluorescent detection of tumor cells.