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Study On Antibiotic Resistance Gene Profiles And Influencing Factors In Accumulated Dust Among Different Urban Public Places In Lanzhou

Posted on:2022-12-21Degree:MasterType:Thesis
Country:ChinaCandidate:T S FengFull Text:PDF
GTID:2491306782483994Subject:Preventive Medicine and Hygiene
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Objective: As a common environmental medium,accumulated dust is widely distributed in the environment and is easily accessible to humans.It is known that accumulated dust contains many toxic and harmful substances.However,there are few studies on antibiotic resistance genes(ARGs)in accumulated dust.ARGs,as a new pollutant,are a potential threat to both the environment and human health.Therefore,this study was conducted to investigate the types,distribution,influencing factors,and potential hosts of ARGs in public place accumulated dust in Lanzhou,for providing theoretical basis of standard application and management of ARGs.Methods: In this study,dust samples were collected from hospitals,shopping malls,parks and boarding schools in Lanzhou in spring and summer respectively,and physicochemical parameters of these dust samples were measured.Then,the microbial communities and ARGs in accumulated dust were investigated.Results:(1)The abundance and diversity of ARGs differed between distinct seasons in Lanzhou.The diversity and abundance of total ARGs,and the abundance of tetracycline and MLSB resistance genes were significantly higher in spring than those in summer.In malls and hospitals,the most abundant ARGs are Sulfonamide resistance genes in dust,followed by Tetracycline resistance genes.In parks and schools,the most abundant ARGs are Tetracycline resistance genes,and the second are Sulfonamide resistance genes.The abundance of total ARGs in accumulated dust did not differ significantly between sampling places,but the abundance of aminoglycoside resistance genes in accumulated dust was higher in hospitals and malls than that in the other sites;the abundance of MLSB resistance genes of accumulated dust was lower in schools and parks compared to malls;and the abundance of multi-drug resistance genes of accumulated dust was higher in hospitals than that in schools and parks.The diversity of ARGs was higher in accumulated dust in malls and hospitals than that in schools and parks.(2)Microorganisms,different places,and mobile genetic elements(MGEs)were primary influencing factors of ARGs in accumulated dust,and the level of ARGs was elevated by the increase of MGEs.(3)Potential pathogenic bacteria,like Streptococcus、Vibrio and Pseudomonas,and high-risk antibiotic resistance genes,such as dfr A1、flo R、tet M-01 and tet M-02,presented in the accumulated dust.In addition,some opportunistic pathogenic bacteria showed significant positive relationships with highrisk ARGs,such as Pseudomonas and tet O-01 presented significantly positive correlation,and Streptococcus was positively correlated to mec A.This implied that these opportunistic pathogens perhaps carried high-risk ARGs.Conclusions:(1)Microorganisms,distinct places,and MGEs may be important influencing factors of ARGs in the accumulated dust,yet different seasons only exert a little effect on ARGs.(2)In Lanzhou,the diversity of dust ARGs in malls is the highest,followed by hospitals,schools,and parks.(3)Some potentially pathogenic bacteria in accumulated dust may be potential hosts for high-risk ARGs.Significance: These results implied that there were potential pathogenic bacteria and ARGs in the accumulated dust,which provided a scientific basis for the clean,disinfection,and use of antibiotics in urban public places.
Keywords/Search Tags:accumulated dust, high-risk antibiotic resistance genes (ARGs), potential pathogenic bacteria, mobile genetic elements(MGEs), potential hosts, human health
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