Preparation And Properties Of Selenizing Dihydromyricetin

Dihydromyricetin（DMY）has certain antioxidant,anti-tumor,and anti-inflammatory effects,while selenium also has antioxidant,anti-cancer,anti-cancer,anti-inflammatory,and immune-enhancing effects.Selenizing DMY was prepared to explore its structure and properties changes and to study its anti-tumor activity in vitro,in order to combine the active functions of DMY and selenium,optimize the physical and chemical properties,and promote the further development and utilization of DMY and selenium resources.Selenizing DMY was prepared with DMY as substrate,Na2Se O3 as selenizing agent,and water and ethanol as solvent environment.Selenizing DMY was evaluated with selenium content and selenium efficiency as the main indicators,and characterized by UV、FTIR、NMR、XRD、TG,etc.On this basis,stability experiments and antioxidant activity experiments were conducted to explore the physicochemical properties of selenizing DMY.The effects of selenizing DMY on the proliferation and migration of A549,HCCLM and HSC-3 cells in vitro were studied.（1）Selenizing DMY was prepared by water as solvent.Based on the selenium efficiency,the orthogonal optimization process was A1B4C4D3,the selenium efficiency was 80.73%±3.28%,and the stability was good.Reducing power and scavenging rate of·OH and O₂^-·free radicals were higher than that of DMY,but the scavenging rate of DPPH free radical was lower than that of DMY.The half inhibition rates of A549,HCCLM,and HSC-3 cells are 36.079,35.784,and 31.705μg/m L,respectively.The scratch healing rate of A549 and HCCLM cells was lower than that of DMY.（2）Selenizing DMY was prepared by water as solvent.Based on the selenium content,the orthogonal optimization process was A4B4C4D3,the selenium content was36.57%±0.54%,and the stability was good.The scavenging rate of·OH free radical was higher than that of DMY.The reducing power and the scavenging rate of DPPH and O₂^-·free radicals are lower than that of DMY.（3）Selenizing DMY was prepared by ethanol as solvent.Based on the selenium efficiency,the orthogonal optimization process was A2B1C3D2E3,the selenium efficiency was 83.23%±2.86%,and the stability was good.The scavenging rate of·OH free radical was higher than that of DMY,the scavenging rate of DPPH free radical is similar to that of DMY,the reducing power and the scavenging rate of O₂^-·free radical are lower than that of DMY.the scratch healing rate of HCCLM and HSC-3 cells was significantly lower than that of DMY.The half inhibition rates of A549,HCCLM and HSC-3 cells were 28.179,42.736 and21.272μg/m L,respectively.The scratch healing rate of HCCLM and HSC-3 cells was significantly lower than that of DMY.（4）Selenizing DMY was prepared by ethanol as solvent.Based on the selenium content,the orthogonal optimization process was A5B1C1D4E3,the selenium content was 17.90%±1.27%,and the stability was good.The scavenging rate of·OH free radical was higher than that of DMY.Reducing power and scavenging rate of DPPH and O₂^-·free radicals were lower than that of DMY.The half inhibition rates of A549,HCCLM,and HSC-3 cells were 15.654,23.936,and 10.421μg/m L,respectively.The scratch healing rate of A549,HCCLM and HSC-3 cells were significantly lower than that of DMY.The characteristic peak of flavonoids in selenizing DMY still exist,and the flavonoids in DMY have not been destroyed by selenization.New C-Se bonds are formed in selenizing DMY.Selenizing DMY has good stability,certain antioxidant activity and good antitumor activity in vitro.