Encapsulation Of Green Tea Polyphenol Nanospheres In PVA/Alginate Hydrogel For Promoting Wound Healing Of Diabetic Rats By Regulating PI3K/AKT Pathway

Objective The purpose of this experiment is to explore whether TPN@H can promote wound healing and it’s mechanism in diabetic rats.Methods1.TPN@H was synthesized and characterized by SEM,FT-IR,UV-vis spectrophotometer,DLS,CLSM,AFM,DSC and thermogravimetric analysis.2.HFF-1 and Ha Ca T cells were selected for cell culture.3.The MTT assay and cell counting kit-8(CCK-8)assay was used to estimate the proliferation of HFF-1 and Ha Ca T cells.4.HFF-1 and Ha Ca T cells migration was detected by scratch wound assay.5.The histological analysis was performed by HE and Masson staining.6.To explore the process of diabetic wound healing.SD rat model of diabetes was established.7.Western blot and immunofluorescence was used to detect the trend of related proteins.Results1.The synthesis of TPN@H was successful,and the results proved that it could be used stably in the following experiment.2.The results of MTT,CCK-8 assay and scratch wound assay indicated that TPN@H could promote the proliferation and migration of Ha Ca T and HFF-1cells.3.The results of HE and Masson staining showed that the TPN@H group had the best wound healing.4.Through the photographs of the wound,the wound closure of TPN@H rats was the fastest.5.The results of immunofluorescence and Western blot indicated that the expression of related proteins of TPN@H group is different.Conclusions In this work,novel tea polyphenol nanospheres(TPN)were synthesized and encapsulated in a PVA /alginate hydrogel(TPN@H).The prepared TPN@H was characterized and applicated in model diabetic rats for promoting wound healing and regulating immune response.Animal experiments and molecular mechanism research proved that TPN@H could promote wound healing of diabetic rats by regulating PI3K/AKT signaling pathway.The result also proved that TPN@H promoted the healing of rat wounds more effectively than TP@H or pure hydrogel.