| Generally,the process of liver regeneration induced by partial hepatectomy(PH)in rats is divided into three phases: the initiation phase(0-6 h after PH),the progressive phase(6-72 h after PH),and the termination phase(72-168 h after PH).The vast majority of hepatocytes in the liver of normal adult mammals are quiescent,but hepatocytes within residual liver quickly enter the cell cycle to compensate for lost liver tissue after liver injury or partial hepatectomy,which is called liver regeneration(liver regeneration,LR).This process is not real regeneration,but a kind of compensatory proliferation.After the liver tissue reaches the original liver weight again,it will not continue to perform cell proliferation,but perform a series of physiological activities such as cell differentiation and apoptosis to restore its original function of the liver,which is regulated by multiple factors including circRNA and miRNA.With the development of bioinformatics,high-throughput detection provides researchers with new directions.We detected the type and expression profile of ceRNA in rat liver regeneration by biological high-throughput technology.Bioinformatics analysis using the rat liver at 24 h after PH found that 2894 differentially expressed mRNAs,147 differentially expressed miRNAs and 70 differentially expressed circRNAs were detected at 0 and 6 h after PH.After determining genes related with the G0 and G1 phase through NCBI and other websites and IPA software,further analysis showed that there were 207 differentially expressed G0 and G1 phase related mRNAs in G0 and G1 phases,and they were predicted to be combined miRNAs by Target Scan and mi Randa websites The number of differentially expressed miRNAs is 87 and the number of differentially expressed circRNAs combined with these 87 miRNAs is 68.We constructed the above-mentioned G0 and G1 phase related differential ceRNA networks by Cytoscape 3.2 software,which found that 135 of 207 mRNA and 87 miRNA constitute 489 miRNA-mRNA axis,and 70 miRNA interact with 68 circRNA to constitute 639 circRNA-miRNA axis,135 mRNAs,70 miRNAs and 68 circRNAs constitute 4012 circRNA-miRNA-mRNA axis.Studies have shown that CEBPb,a member of the CCAAT enhancer binding protein family,can regulate afp,a2 m,gsta2,cat,il6,col1a2 and col2a1,etc.,and our team has found that CEBPb expression is upregulated early after PH in rat liver.In this article,we continue to study the related effects of CEBPb-mediated circRNA-miRNA on G0 / G1 phase in rat liver regeneration,thus further revealing that the circRNA-miRNA-CEBPb mRNA axis regulates G0 and G1 phases of liver cells in rat liver regeneration molecular mechanism.To this aim,we screened the ceRNA high-throughput detection data of rat liver regeneration,and defined the G0 and G1 phase related genes through NCBI and other websites and software IPA,and found the downstream target genes regulated by CEBPb through CDB and other websites,and through Target Scan and other websites to predict the miRNA bound by CEBPb mRNA,then using usedsoftware such as mi Randa to predict the circRNA bound with the miRNA.Their expression patterns and molecular mechanisms were analyzed by bioinformatics method,and they were preliminarily screened out 11 species circRNAs Combined with 3 target miRNAs.And the interaction of the 7 downstream target genes regulated by CEBPb Through the circna miRNA cebpb mRNA axis,the G1 phase of G0 phase in rat liver regeneration plays a key role. |
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