Effects Of Hepatic Branch Of Isolated Vagus Nerve On Blood Glucose And Lipid Level In Rats And Its Relationship With MTOR Expression Level In Skeletal Muscle

Objective:to investigate the effect of removing the vagus nerve in rat liver on blood glucose and lipid level and its relationship with the expression level of mTOR in skeletal muscle,so as to provide experimental basis for the study of the neuromodulation mechanism of glucose and lipid metabolism.Methods:Based on the method of surgery without the rat model of hepatic branch of the vagus nerve as the experimental group(n = 30),the other Settings of vagus nerve liver probe control group(n = 30)and do not do any processing of the control group(n = 30),the observation group rats mental status and diet,urine,etc.Generally,after 2 weeks,4 weeks,6 weeks rats from each group randomly selected 10,only use electronic weighing rat body weight,use of automatic biochemical analyzer test each state on an empty stomach blood sugar of rats(Fasting plasma glucose,FPG,Total cholesterol(TC),Triglycerides(TG),High density lipoprotein(HDL),Low density lipoprotein(LDL)levels,and fully automatic electrochemilescence immunoanalyzer(ELECSYSC)were used to detect fasting plasma Insulin(INS)and C peptide of rats in each group.CP)and Cortisol(Cortisol,COR)level,glycogen dyeing liquid(Digestion periodate shev reaction,D-PAS,enzyme Digestion method)to detect each rat liver glycogen content on an empty stomach,Immunohistochemical technique(Immunohistochemical techniques,IHC)testing each rat skeletal muscle rapamycin target protein(Mammalian target of rapamycin,mTOR)expression level,The levels of mTOR and Phosphorylated mammalian target of rapamycin(p-mtor)were determined by Western blot(WB).Results:1.On the day after the operation,the rats in the experimental group and the sham operation group had decreased diet,drinking water,defecation and urination,and were in poor mental state.They gradually recovered within 3 days.Compared with the control group and the sham group,the rats in the experimental group lost weight at 2weeks,4 weeks and 6 weeks(P<0.05).There was no significant difference in postoperatively weight between the sham group and the control group(P>0.05).2.Compared with the control group and the sham group,the fasting blood glucose(FPG)level of the experimental group decreased 2 weeks after the operation,while the fasting blood glucose(FPG)level increased 6 weeks after the operation(P<0.05),and there was no significant difference 4 weeks after the operation(P>0.05).There was no significant difference in fasting glucose(FPG)level between the control group and the sham group at all time points(P>0.05).3.Compared with the control group and the sham group,the triglyceride(TG)level of the experimental group increased and high-density lipoprotein(hdl-c)level decreased 6 weeks after the operation(P<0.05),and there was no significant difference 4 weeks after the operation(P>0.05).There was no significant difference in serum lipids between the sham group and the control group at all time points(P>0.05).4.Compared with the control group and the sham group,the level of insulin(INS)in the experimental group was significantly increased 2 weeks after the operation(P<0.05),and there was no significant difference between 4 weeks and 6 weeks after the operation(P>0.05).There was no significant difference in c-peptide,cortisol(COR)and insulin(INS)levels between the sham group and the control group at each time point(P>0.05).5.Compared with the control group and the sham group,the liver glycogen content of the experimental group increased 2 weeks after the operation,while decreased 6 weeks after the operation(P<0.05),and there was no significant difference 4 weeks after the operation(P>0.05).There was no significant difference in liver glycogen content between sham operation group and control group at each time point(P>0.05).6.Compared with the control group and the sham group,the expression levels of mTOR and p-mtor in skeletal muscles in the experimental group increased 6 weeks after the operation(P<0.05),and there was no significant difference between 2 weeks after the operation and 4 weeks after the operation(P >0.05).There was no significant difference in mTOR and p-mtor levels between the sham group and the control group at each time point(P >0.05).Conclusions:1.Isolated hepatic branch of vagus nerve in rats can cause abnormal glucose and lipid metabolism and glycogen content in liver.2.Metabolic abnormalities caused by the hepatic branch of the vagus nerve in isolated rats may be related to changes in insulin level and increased expression of mTOR in skeletal muscle.