Study On The Prognosis Of MiR-106b-5p Detected From FFPE Sample In Clear Cell Renal Cell Carcinoma

Background: Kidney cancer is a common malignant tumor in our country,which seriously threatens people’s health.The incidence and death rate of kidney cancer in our country are increasing year by year.Because of the insignificant symptoms in the early patient and the high metastasis and recurrence rates,the early diagnosis rate and 5-year survival rate are still low.It’s also found that the 5-year survival rate varies with the progression of the tumor.If metastasis occurs,the 5-year survival rate will be further reduced to less than 10%.It can be seen that accurate evaluation of the prognosis of renal cancer patients is important for achieving personalized and precision treatment.However,there are no clinically applicable prognostic markers for renal cancer.Micro RNA(miRNA)is a short RNA molecule composed of approximately 22 nucleotides,which accounts for approximately 3% of the human genome.The potential role of miRNAs in cancer is to participate in the regulation of tumor cell proliferation,differentiation and apoptosis by controlling the expression of tumor suppressor genes and oncogenes.miRNAs have also been confirmed to be related to the diagnosis,recurrence prediction and prognosis of a variety of cancers.The current methods used to detect miRNA include quantitative PCR(q PCR),in situ hybridization,microarray,and RNA sequencing.miRNA can be detected in a variety of biological samples such as cells,tissues and body fluids(such as serum,plasma,tears and urine).miRNA has the characteristics of stability and sensitivity,which is also easy to be detected.In recent years,more and more miRNAs have been found to be related to the prognosis of kidney cancer.herefore,miRNAs are expected to become prognostic markers of renal cancer.Objective: TIn this study,we detect the expression level of miR-106b-5p in formalin-fixed paraffin-embedded(FFPE)specimens of renal cancer in tumor tissues and adjacent normal tissues by use reverse transcription real-time quantitative polymerase chain reaction(RT-q PCR).And then,we explore the correlation between the expression level of miR-106b-5p and the prognosis of renal cell carcinoma patient,and the potential of miR-106b-5p as a prognostic marker of renal cancer.Methods: We collected formalin-fixed paraffin-embedded(FFPE)specimens of tumor tissues and normal tissues adjacent to cancer in 284 cases of postoperative renal cancer patients through certain inclusion/exclusion criteria.The expression of miR-106b-5p was examined by Reverse Transcription Real-time Quantitative Polymerase Chain Reaction(RT-q PCR),followed by correlation analysis with clinicopathological features and prognosis.Patient survival analysis was determined by the Kaplan-Meier methodusing the log-rank test was used for patient survival analysis,and the univariate and multivariate analysis was determined by the Cox’s regression model.Results: There was no significant correlation between the expression level of miR-106b-5p and age,gender,tumor size or tumor stage.Kaplan-Meier analysis showed that compared with patients with low miR-106b-5p expression,patients with high miR-106b-5p expression had significantly shorter overall survival(p = 0.001).Univariate and multivariate analysis believe that the expression of miR-106b-5p is an independent risk factor for predicting the prognosis of renal cancer patients.In addition,analysis of data from 506 cases of kidney cancer from The Cancer Genome Atlas database(TCGA)also concluded that the high expression of miR-106b-5p predicts a poor prognosis in patients with kidney cancer.Conclusions: The high expression of miR-106b-5p in formalin-fixed paraffin-embedded(FFPE)specimens is an independent factor predicting the poor prognosis of kidney cancer patients.