| Objective: Explore the effect of Qigu capsule combined with alendronate on osteoporosis model rats.Methods: Select 140 three-month-old SD female rats,about 250±20g,of which 120 were randomly selected to make a pathological model of postmenopausal osteoporosis,and the remaining 20 were the blank group(group A).120 rats were randomly divided into six groups,model group(group B),alendronate treatment group(group C),Qigu capsule treatment group(group D),low-dose Qigu+alendronate group(group E),middle-dose Qigu+alendronate group(group F)and high-dose Qigu+alendronate group(group G),a total of seven groups,each Group of 20.The rats in group A and B were given intragastrically with double distilled water daily,and the remaining groups were given intragastrically according to the grouping category(Stilbene: 900mg/kg/d,A: 2mg/kg/d;100g/ml)for 12 consecutive weeks.At the 4th,8th and 12th weeks,1/3 of the rats were taken to test the serum E2,PINP,and β-CTx concentrations;dual-energy X-rays were performed to detect the bone density of the L4 vertebra,and the bone strength tester to detect the maximum bone strength of the right femur Load and make pathological sections of the left femur for HE staining,and observe the changes of trabecular bone under an optical microscope.Results:(1)Comparing the E2 level of the blank group and the model group,the level of the model group was significantly reduced after the model being established in 4 weeks(P<0.01).In the 8th week,compared with the Allen group and the low-dose group,the high-dose E2 level was significantly increased(P<0.01,P<0.05);in the 12th week,compared with the Qigu group,Alun group and the low-dose group,the E2 level of the high-dose group was significantly increased(P<0.05,P<0.01,P<0.05).Compared with the three groups in the low,medium and high dose(Qigu + Alan)groups,the E2 level increased with the increase of the dose in the 4th,8th and 12th weeks,which was concentration-dependent.(2)Compared with the blank group,the model group was significantly improved in each period,and the difference was statistically significant(P<0.01).The β-CTx levels of each drug intervention group were lowered in the 4th week compared with the model group(P>0.05).In the 8th and 12th weeks,the middle and high-dose groups were compared with the model group and low-dose groups,there were significant differences between the groups(P<0.05,P<0.01).(3)Comparing the model group with the blank group,the PINP level was significantly reduced in 4,8 and 12 weeks(P<0.01).Compared with the model group,the PINP level of each drug intervention group increased in the 4th week(P>0.05).The 8th and 12th week PINP level was significantly higher than model group(P<0.01).In the 8th week,the PINP level of the high-dose group was significantly higher than that of the Allen group;and in the 12th week,it was significantly higher than that of the Allen group and the low-dose group.The difference between the groups was obvious.(4)In the 4th week,the bone mineral density of L4 vertebra in the model group was slightly lower than that of the blank group(P>0.05).In the 8th and 12th weeks,the bone mineral density of the model group was compared with that of the blank group,it was decreased.And the difference was statistically significant(P<0.05).The bone mineral density of each drug intervention group increased compared with the model group at the 4th and8 th week(P>0.05);in the 12th week,the medium and high-dose group was statistically compared with the model group academic significance(P<0.05,P<0.01).(5)In the 4th week,the maximum load of the right femur of the model group was reduced compared with other groups(P>0.05).In the 8th and 12th weeks,the maximum load of the model group was compared with the blank group,it was significantly reduced,and the difference was statistically significant(P<0.01).In the 4th and 8th week,the maximum load of the right femur of each drug intervention group was significantly increased compared with the model group(P>0.05);in the 12th week,the difference was statistically significant(P<0.05,P<0.01).(6)After HE staining,under optical microscope observation,the trabecular bone tissue of the left femur of the blank group is full,densely arranged,and the trabecular bone structure is complete.The trabecular bone tissue of the rat PMOP model became thinner,the trabecular bone was broken and discontinuous,and the bone marrow cavity was significantly larger than that of the blank group.After intragastric administration in each drug intervention group,the trabecular bone tissue gradually changed,the shape gradually became fuller,the arrangement became dense,and the shape and structure gradually became continuous and complete,and the bone marrow cavity gradually became thinner.Conclusion: Qigu capsule combined with alendronate can increase the level of E2,improve the serum bone metabolism indexes of OP rats,increase the level of PINP,and inhibit the level of β-CTx.It can increase the bone density and strength of OP rats caused by the decrease of estrogen and improve the morphological performance of bone tissue. |
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