Flow Cytometric Analysis Of T/NK Cells In Lymphoma And Autoimmune Diseases

Purpose:Through detection of the proportion of peripheral blood lymphocyte subsets and the fluorescence intensity of CD molecular in lymphoma,autoimmune disease,and healthy controls,to compare the differences in the subsets of peripheral blood lymphocytes between the two groups of diseases.Explore the value of flow cytometry in the differential diagnosis of autoimmune diseases and lymphoma.Methods:A total of 57 patients with newly diagnosed lymphoma in the“Second Affiliated Hospital of Chongqing Medical University”were enrolled and divided into B-cell non-Hodgkins lymphoma group（B-NHL）and T/NK-cell non-Hodgkins lymphoma group（T/NK-NHL）and Hodgkins lymphoma group（HL）;a total of 34 patients with treated autoimmune diseases were included and divided into rheumatoid arthritis group（RA）and systemic lupus erythematosus group（SLE）;17 cases of healthy control group were included.A 10-color flow cytometer was used to detect the proportion of T cells,NK cells and the average fluorescence intensity of the peripheral blood lymphocyte subsets of the collected cases,and the data was statistically analyzed by analysis of variance and non-parametric tests.Results:1.We found that lymphoma and autoimmune diseases differed in the proportion of peripheral blood T cells and NK cell subsets.The proportion of CD4⁺CD25⁺cells in the peripheral blood of the B-NHL group was higher than that of the SLE group;the peripheral blood CD56^dimCD16^brightcells of the B-NHL group The proportion of CD56^dimCD16^brightcells in the T/NK-NHL group was higher than that of the SLE group;the proportion of CD56^dimCD16^dimcells in the peripheral blood of the B-NHL group was higher than that of the SLE group,and the differences were statistically significant（p<0.05）;2.There are differences in the average fluorescence intensity（MFI）of CD56 in NK cells.The average fluorescence intensity of CD56 of NK cells in the B-NHL group is higher than that of the RA group and the control group,and the average fluorescence intensity of CD56 of NK cells in the RA group is lower than that of the control group;The average fluorescence intensity of CD16 of NK cells in the B-NHL group was lower than that of the RA group and the control group.The average fluorescence intensity of CD16 of NK cells in the RA group was higher than that of the control group,and the difference was statistically significant（p<0.05）.Conclusion:There are differences in the proportion of peripheral blood lymphocyte subsets and the average fluorescence intensity of newly diagnosed lymphoma,treated autoimmune diseases,and healthy people.Among them,the measurement of the average fluorescence intensity of NK cells CD16 and CD56 can be used as a marker for differential diagnosis by flow cytometry.