Expression And Clinical Significance Of MiR-155-5p,caspase-1mRNA,IL-1βmRNA,IL-18mRNA In Patients With Lupus Nephritis

Objective:This experiment detects the expression of miR-155-5p and inflammatory factors(Caspase-1mRNA,IL-1βmRNA,IL-18mRNA)in the blood and urine of patients with lupus nephritis,and analyzes the relationship between miR-155-5p and Caspase-1mRNA,IL-1βmRNA and IL-18mRNA.At the same time,it analyzes the relationship between miR-155-5p,Caspase-1mRNA,IL-1βmRNA,IL-18mRNA and general clinical data to explore the relationship between miR-155-5p and inflammatory factors in patients with lupus nephritis which studies its possible mechanism of action.Methods:(1)35 samples of healthy blood and fresh mid-morning urine samples were collected from April 2020 to November 2020 in the Affiliated Hospital of Youjiang National Medical College for Nationalities.In addition,20 samples of healthy blood and fresh mid-morning urine samples were collected as a control group.(2)The expression of miR-155-5p,Caspase-1mRNA,IL-1βmRNA,IL-18mRNA,IL-18 BPmRNA in peripheral blood mononuclear cells and urine sediment cells were measured by RT-PCR.(3)The expression of miR-155-5p and inflammatory factors(Caspase-1mRNA,IL-1βmRNA,IL-18mRNA)in PBMC and urinary sediment cells of lupus nephritis patients were analyzed by correlation analysis.(4)The general clinical data of lupus nephritis patients were collected,and then analyzed with their correlation with miR-155-5p and inflammatory factors(Caspase-1mRNA,IL-1βmRNA,IL-18mRNA).Results:(1)The expressions of miR-155-5p,Caspase-1mRNA,IL-1βmRNA,IL-18mRNA and IL-18 BPmRNA in PBMC of lupus nephritis patients were higher than those in control group(P<0.01).The expressions of Caspase-1mRNA,IL-1βmRNA,IL-18mRNA and IL-18 BPmRNA in urine sediment cells were higher than those in the control group(P<0.01),while the expression of miR-155-5p was lower than that in the control group(P<0.01).(2)The expression of miR-155-5p in PBMC of lupus nephritis patients is positively correlated with the expression of Caspase-1mRNA,IL-1βmRNA and IL-18mRNA(P<0.01).The expression of miR-155-5p in urine sediment cells has no significant correlation with the expression of Caspase-1mRNA,IL-1βmRNA and IL-18mRNA,and the difference is not statistically significant(P>0.05).(3)The expression of miR-155-5p in urine sediment cells of lupus nephritis patients is positively correlated with C3(P<0.05).Caspase-1mRNA,IL-1βmRNA and IL-18mRNA in PBMC and urine sediment cells of lupus nephritis patients are negatively correlated with C3(P<0.05).There is a positive correlation between IL-18mRNA and SLEDAI in PBMC of lupus nephritis patients(P<0.05).(4)The AUC values of miR-155-5p,Caspase-1mRNA,IL-1βmRNA and IL-18mRNA in PBMC of patients with lupus nephritis are 0.786,0.913,0.885 and 0.881 respectively,which are statistically significant compared with 0.5(P<0.05).The AUC value of miR-155-5p in urine sediment cells of lupus nephritis patients was 0.240,which was not statistically significant compared with 0.5.The AUC values of Caspase-1mRNA,IL-1βmRNA and IL-18mRNA in urine sediment cells of patients with lupus nephritis are 0.851,0.815 and 0.899 respectively,which are statistically significant compared with 0.5(P<0.05).Conclusion:(1)The expression levels of miR-155-5p,Caspase-1mRNA,IL-1βmRNA and IL-18mRNA are increasing in PBMC of lupus nephritis patients.(2)The expression of miR-155-5p is positively correlated with the expression of inflammatory factors(Caspase-1mRNA,IL-1βmRNA and IL-18mRNA)in lupus nephritis PBMC,and there may be a regulatory relationship between miR-155-5p and Caspase-1mRNA,IL-1β and IL-18.(3)Inflammatory factors(Caspase-1mRNA,IL-1βmRNA,IL-18mRNA)are correlated with clinical inflammatory activity indicators.miR-155-5p and inflammatory factors have higher diagnostic accuracy for lupus nephritis,and miR-155-5p and inflammatory factors can be used as new inflammatory markers for lupus nephritis.