Difference Of Oral Microbiota Composition In Patients With Chronic Kidney Disease At Stage 5 Hemodialysis

Objective:In 2007,the National Institutes of Health(NIH)launched the Human Microbiome Project(HMP)to uncover the link between microbes and Human disease.Since then,the study of human microbiome in various countries has reached an unprecedented hot level.In 2008,NIH National Institute of Dental and Craniofacial Research(NIDCR)launched the Human Oral Microbiome Database(HOMD)program,which is the first programmatic description of the Human Microbiome and provides a tool for understanding the role of the Microbiome in health and disease.The main goal of HOMD is to provide comprehensive information on the communities of hundreds of human oral prokaryotic species.In 2015,the "National Individual Microbiome Detection Project" was launched again,aiming to take the oral,skin and intestinal microbiome as the main research targets,to carry out clinical transformation of the research results of the "Human Microbiome Project",and to find more accurate disease early warning molecules from the "second genome".To analyze the diversity of oral flora and metabolites in hemodialysis patients and control group by high throughput 16 S r RNA sequencing and metabonomics methods,To provide a theoretical basis for the adjuvant treatment of chronic kidney disease by regulating oral flora.Methods:1.With the informed consent of the research subjects,25 hemodialysis patients in Fujian Province Hospital were selected,and 25 healthy person from the physical examination center of the hospital during the same period were selected as the control group,and their saliva was taken as the study sample.2.To study the diversity of oral flora of hemodialysis patients and control group,by using high-throughput sequencing and metabonomics methods,To investigate the differences in oral flora diversity and metabolites between hemodialysis patients and control group.Results:1.It is no significant difference betweem hemodialysis patients group and healthy control group in gender,age and body mass index(P > 0.05).2.To use usearch software for quality control of the reads to the chimera,with condition of 0.97 in similarity,all samples were obtained 1336 different OTU database,OTU and 16 S have been proved the species,the species annotation for each OTU,1species in the community level,gate level 22 species,45 species at class level,the level of the 71 species,122 species belonging to the family level,the level of the 189 species.3.Statistical analysis of bacteria showed the structure of oral flora in each group was different.Comparison of the healthy control group,the hemodialysis patients group indicated primary insomnia group of oral bacteria was the greatest contribution,and the species were: Leptotrichia,Capnocytophage,rynebacterium,rkholderia,oribacter,lobaculum,ospira,linibacterium,robacterium.The healthy control group had the greatest contribution to the differences were: Haemophilus,gregatibacter,gibacterium,tinobacillus,cherichia,ptothrix,tterella.Conclusion:1.There were abundant symbiotic communities in the oral cavity,and there were significant differences in oral flora composition between hemodialysis patients and healthy controls group.Comparison of the healthy control group,the hemodialysis patients group indicated primary insomnia group of oral bacteria was the greatest contribution,and the species were: Leptotrichia,Capnocytophage,rynebacterium,rkholderia,oribacter,lobaculum,ospira,linibacterium,robacterium.The healthy control group had the greatest contribution to the differences were: Haemophilus,gregatibacter,gibacterium,tinobacillus,cherichia,ptothrix,tterella.2.Hemodialysis patients are associated with oral flora disorders.Oral flora disorders induce microinflammatory state,cause systemic inflammatory response,and then lead to immune disorders,thus accelerating the progression of chronic kidney disease and the generation of cardiovascular complications.With the development of CKD,oral oral flora disorders are promoted,and they effect each other,resulting in a poor prognosis.It is suggested that the disease progression and complications of chronic kidney disease can be delayed by regulating oral flora.