| Objective: To observe the effect of allogeneic platelet-rich fibrin(PRF)on wound healing in diabetic rats,and to explore its effects on wound angiogenesis,cell proliferation and vascular endothelial growth factor content,and to explore its mechanism of promoting wound healing in diabetic rats.Methods: A total of 120 rats were selected for the experiment,of which 40 were used to prepare allogeneic PRF,80 rats to make diabetic rat model.Sixty-four diabetic rats were randomly divided into two groups: 32 diabetic rats were set up as the experimental group and 32 diabetic rats as the control group.A 1.5 × 1.5cm wound was made in the center of the back of diabetic rats.The wounds in the experimental group were treated with PRF and vaseline gauze extracted from rat allogeneic blood,while those in the control group were treated with vaseline gauze only.The wound healing of rats in each group was observed and photographed on the 3rd,7th and 14 th day,respectively,and the wound healing rate of each group was calculated.After 3 days,7 days and 14 days,8 rats were killed to observe the histological changes by HE staining.Immunohistochemical staining was performed for CD34 staining,proliferating cell nuclear antigen(PCNA)staining and vascular endothelial growth factor(VEGF)staining.The expressions of CD34,PCNA and VEGF were compared between the two groups to prove the role of allogeneic PRF in wound healing of diabetic rats.Results: The wound healing rate of the experimental group was higher than that of the control group at 3d,7d and 14 d,and the difference was statistically significant(P < 0.05).The wound healing time in the experimental group was shorter than that in the control group,and there was significant difference between the two groups(P < 0.05).Histological observation of HE staining showed that the healing condition of the experimental group was better than that of the control group.The results of immunohistochemical CD34 staining showed that the angiogenesis of the wound in the experimental group was significantly higher than that in the control group at 3d,7d and 14d(P < 0.05).The results of immunohistochemical PCNA staining showed that the number of cells in the proliferative phase in the experimental group was significantly higher than that in the control group at 3d,7d and 14d(P < 0.05).The results of immunohistochemical VEGF showed that the expression of vascular endothelial growth factor(VEGF)in the experimental group was significantly higher than that in the control group on the 3rd,7th and 14 th day.Conclusion: Allogeneic PRF can promote wound healing and improve wound healing rate in diabetic rats.The mechanism is related to increasing the number of wound microvessels,promoting wound cell proliferation and increasing the expression of VEGF in wound tissue. |
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