Studies On The Ethanol Extraction Process From Leaves Of Rehmannia Glutinosa Libosch. And Its Effects On ARF Mouse

Orthogonal design was utilized to optimize the preparing conditions of ethanol extracts of leaves of Rehmannia glutinosa Libosch.The optimum extraction process was to extract three times using tenfold 50%ethanol and 1.0 h per time.The active carbon was utilized to purify the ethanol extract from leaves of Rehmannia glutinosa Libosch.The purification conditions were obtained as follow:active carbon(46 times of ethanol extract)was added to the water solution of ethanol extract(10mg/ml),and placed 12h,then subjected to chromatography column,and eluted with 5BV water and 3BV 30%ethanol successively.The catalpol content of ethanol extract was raised from 6.19%to 15.30%after purification.The macroporous absorption resin was utilized to purify the ethanol extract from leaves of Rehmannia glutinosa Libosch further.The purification conditions were obtained as follow:the water solution of ethanol extract(catalpol concentration:0.5mg/ml)was subjected to H103 macroporous absorption resin at the velocity of 1BV/h,and eluted with 5BV water and 4BV 10%ethanol successively at the velocity of 3BV/h.The catalpol content of ethanol extract was raised from 15.30%to 46.60%after purification.Two acute renal failure mouse models were established,which were induced with aristolochic acid A and glycerol respectively.The anti-acute renal failure effects of ethanol extract from leaves of Rehmannia glutinosa Libosch were studied on the mouse models.The results indicated that the ethanol extract had the effects of anti-acute renal failure mouse models,and catalpol was an active ingredient,and there were other ingredients in ethanol extract from leaves of Rehmannia glutinosa Libosch,which could increase the effects of anti-acute renal failure mouse models in addition to catalpol.