Effect Of Shikonin On Epithelial Mesenchymal Transition Of Cervical Cancer Hela And C33a Cells

Objective:In this study,shikonin was applied to cervical cancer Hela and C33a cells to investigate the effects of shikonin on the proliferation and epithelial-mesenchymal transition of cervical cancer Hela and C33a cells,and to seek early postoperative cervical cancer and post-operative cervical cancer after radiotherapy and chemotherapy.The maintenance of traditional Chinese medicine seeks to explore the Possible therapeutic effects of shikonin,and provides an experimental basis for the development of the possibility of Chinese medicine in the adjuvant treatment of cervical cancer after standard Western medicine treatment.Method:1.MTT assay to show the effect of shikonin on the proliferation inhibition of cervical cancer Hela and C33a cells.2.Flow cytometry to detect the periodic distribution of shikonin on cervical cancer Hela and C33a cells.3.Scratch test to determine the effect of shikonin on the migration ability of cervical cancer Hela and C33a cells.4.Transwell method for detecting the invasive ability of shikonin on cervical cancer Hela and C33a cells.5.WB method was used to detect the expression of E-cadherin,vimentin and snail in epithelial-mesenchymal transition proteins of cervical cancer Hela and C33a cells.Result:The results of MTT showed that compared with the blank group,the shikonin group significantly inhibited the proliferation of cervical cancer Hela and C33a cells,and the difference was statistically significant(P<0.01).Flow cytometry results showed that compared with the blank group,the shikonin group blocked the cycle of Hela and C33a cells in the G0/G1 phase,and the difference was statistically significant(P<0.01).The results of the scratch test showed that compared with the blank group,the shikonin group effectively reduced the migration ability of cervical cancer Hela and C33a cells,and the difference was statistically significant(P<0.01).The results of Traswell showed that compared with the blank group,the shikonin group effectively Prevented the invasion of cervical cancer Hela and C33a cells,and the difference was statistically significant(P<0.01).The results of WB showed that compared with the blank group,the shikonin group up-regulated E-cadherin in cervical cancer Hela cells,and down-regulated the expression of vimentin and snail protein,the difference was statistically significant(P<0.01).Similarly,shikonin up-regulated the expression of E-cadherin in C33a cells and down-regulated the expression of vimentin and snail protein compared with the blank group(P<0.01).Conclusion:Shikonin inhibits the invasion and migration of cervical cancer Hela and C33a cells,which may be through up-regulating the expression of E-cadherin in epithelial-mesenchymal transition and down-regulating the expression of vimentin and snail proteins.