Study On Extraction Process Of Total Flavonoids From Phellinus Igniarius And PH Sensitive Liposomes Of Morin

Nanomaterials with target properties have many potential for anticancer applications.Morin plays an important role in inflammation,anticancer,and aging.However,Morin has extremely low water solubility,which is a major challenge for pharmaceutical use.In this paper,the extraction process of total flavonoids from Phellinus igniarius was optimized.Combined with single factor experiment and orthogonal experiment,the optimal process for extracting total flavonoids from Phellinus igniarius by ultrasonic method was temperature 20℃,ethanol concentration 60%,soaking time 24h,ultrasonic The time was 60 min,and the highest total flavonoid extraction rate was 0.67%.The crude mulberry flavonoids were found to exist in the ethyl acetate extract phase by extraction reaction and thin layer chromatography.The extract was further purified by polyamide resin to obtain the Morin sample.The purified sample and the mordant standard were subjected to high performance liquid chromatography(HPLC).The sample was found to have similar retention time and purity compared with the standard.There are a few differences.A new type of anticancer nanoparticle,Au nanoparticle-modified pH-sensitive liposome with target properties(Apt-Au@MSL),is synthesized in this paper.The Apt-Au@MSL exhibited excellent monodispersity and tumor-targeting properties.And the polarity of morin is changed and the antitumor activity is enhanced.We screened our model cancer cell through MTT assay,and we foundSGC-7901 cells can be effective suppression proliferation.The fluorescent and flow cytometry assays confirmed that Apt-Au@MSL could serve as effective anticancer activity material and induced the apoptotic in vitro.The target properties of Apt-Au@MSL were confirmed through TEM images.Through results in vivo demonstrated that the administration of Apt-Au@MSL could inhibit tumor growth in xenograft mouse models.The H&E staining and TUNEL assay further confirmed that Apt-Au@MSL can promote tumor apoptosis.Both blood biochemistry tests and H&E staining suggested that these materials have negligible acute toxicity and good biocompatibility in vivo.The powerful Apt-Au@MSL function could as a target-based anticancer material for future clinical cancer treatment.