Suppression Effect Of Plumbagin On Chronic Periodontitis Via The Down-regulation Of TNF-α,IL-1β And IL-6 Expressions:in Vivo And Vitro Studies

ObjectiveChronic periodontitis(CP)is one of the most common oral diseases which causes alveolar bone absorption and tooth loss in adults.In this study,we aimed to remedy chronic periodontitis with Plumbagin(PL),one widely-investigated pharmacologic drug derived from traditional Chinese medical herb.MethodsThe anti-inflammation property of PL is evaluated in TNF-α-induced human periodontal ligament stem cells(PDLSCs)and ligated and Porphyromonas gingivalis(Pg)stimulated Sprague-Dawley rats(SD rats).Proinflammatory cytokines TNF-α,IL-1β and IL-6 were evaluated for analysis of periodontitis pathogenesis.ResultsResults indicated that PL inhibits CP progression by down regulating the expressions of TNF-α,IL-1β and IL-6 in both in vivo and vitro studies(p<0.01).Phosphorylation of the mitogen-activated protein kinase(MAPK),nuclear factor-κB(NF-κB)and JAK/STAT signaling pathways was suppressed after treatment of PL in PDLSCs in western blot(p<0.001).Chemotaxis migration of macrophages was restrained due to reduction of CCL-2 and CCL-5(p<0.01).In the animal experiment,deceleration in bone destruction was observed through Alveolar Bone Loss(ABL)and H&E staining results(ABL: p<0.01).ConclusionWith the given data,conclusion could be made that PL suppressed CP by down regulating the expressions of TNF-α,IL-1β and IL-6,and that the progress was inhibited through MAPK,NF-κB and JAK/STAT signaling pathways in vitro studies,thus indicating its anti-inflammation property against CP.