Correlations Between Human Papillomavirus Infection And The Pathogenesis And Prognosis Of Laryngeal Squamous Cell Carcinoma

Objective Human papillomaviruses(HPVs)belonging to the papillomaviridae family are non-enveloped viruses that are comprised of a double-stranded circular DNA within an icosahedral capsid.Head and neck squamous cell carcinoma(HNSCCs)affect 600,000 patients per year worldwide.Smoking and alcohol abuse are implicated in the rise of HNSCC in past decades,but the role of human papillomavirus(HPV)is emerging as an important factor in the rise of HNSCC affecting non-smokers in developed countries.This strong correlation found between human papillomavirus(HPV)infection and the increasing incidence of head and neck squamous cell carcinoma,especially oropharyngeal squamous cell carcinoma(OPSCC)has been confirmed by many clinical tests,while the correlation between HPV infection and the incidence of laryngeal squamous cell carcinoma remains undiscovered.We aim to determine whether human papillomavirus infection status is related to prevalence and prognosis of laryngeal squamous cell carcinoma(LSCC)patients.Methods A retrospective cohort study was applied on a total of 252 LSCC patients were enrolled in Department of Head and Neck Surgery,Renji Hospital of Shanghai Jiaotong University School of Medicine from 2004 to 2013.Paraffin-embedded tissues of all patients were cut as 4 μm slices.P16 immunohistochemistry and fluorescence quantitation PCR High Risk HPV DNA test was applied to detect the HPV infection status respectively in 252 specimens.Long-term survival rates were calculated using the Kaplan–Meier method.Results 6.0%(15/252)of LSCC patients were HPV DNA positive.All the HPV DNA positive patients were p16 positive,93.3%(14/15)of the HPV DNA positive patients were HPV type 16 and the rest was HPV type 18.The 3-year overall survival rates were 67.0% for all 252 patients,77.0% for HPV DNA positive patients,65.0% for HPV DNA negative patients(P=0.25).Conclusions HPV has low prevalence in Chinese LSCC people.HPV infection status is associated with LSCC but may not be an available prognostic factor for LSCC.The expression of p16 has limited significance for the prognosis of LSCC patients.