SIRT3 Deacetylated And Increased Citrate Synthase Activity In PD Model

SIRT3 have been found to be neuroprotective in many neurological diseases,but its detail mechanism is only partially understood.In this study,MPP~+was used to treat SH-SY5Y cells as the cellular model of PD to test the role of SIRT3 and the mechanism may be involved in.We focused on the changes and relationship between SIRT3 and the key mitochondrial enzymes citrate synthase(CS)and isocitrate dehydrogenase 2(IDH2).And also we detected the activities of CS and IDH2.We found that MPP~+decreased the expression of SIRT3,CS and IDH2.And our results showed that the enzymatic activities of CS and IDH2 were significantly reduced in MPP~+treatment cells,while protein acetylation of CS and IDH2 increased.However overexpressed-SIRT3partially at least,made the increase of CS activity and decrease of CS acetylation.IDH2 did not show the same changes.The study suggested that SIRT3 could deacetylate and activate CS activity.Hence,we conclude that SIRT3 exhibits neuroprotection via deacetylating and increasing mitochondrial enzyme activities.Our results may provide new strategies for new drug research and development for PD.