Effects Of Stilbene On Biological Behavior Of Gastric Ccncer Cells And Hepatotoxicity

1 The Effect of THSG on Biological Behavior of Gastric Cancer Cells.Objective To investigate the biological effects of THSG on gastric cancer cells.Research methods Different concentrations of THSG(0.6m M,0.7m M,0.8m M,0.9m M,1.0m M,1.1m M,1.2m M,1.3m M,1.4m M)In gastric cancer MGC803 and MGC7901 cells 24 hours,cck8 method to detect the proliferation of two gastric cancer cells,after the IC50,based on IC50 to select the concentration for follow-up experiments;TRANSWELL detects the migration and invasion of two gastric cancer cell cells,and the flow cycle analyzes the cycle changes of two gastric cancer cells.Results CCK8 results showed that THSG concentration dependence significantly reduced the survival rate of in vitro gastric cancer MGC803 and MGC7901 cells,which were statistically significant(P < 0.05);Both significantly decreased,the number of clones decreased significantly,and the scratch experiment showed that,compared to the control group,THSG treatment significantly reduced the healing rate of the two cells at 12 h and 24 h,and was statistically significant(P < 0.05).Transwell experimental results showed that compared with the control group,THSG treatment significantly reduced the number of migrations,the number of attacks,and statistically significant(P <0.01).Flow cycle monitoring analysis showed that the proportion of G0/G1 and S cells of two gastric cancer cells decreased significantly compared with the control group(P < 0.01),and the proportion of Cells in G2/M period decreased significantly(P < 0.05).Conclusion THSG inhibits the proliferation,cloning,migration and invasion of gastric cancer cells.2 THSG Induces Apoptosis and Autophagy of Gastric Cancer Cells and Mechanism StudyObjective To investigate whether THSG induces apoptosis and autophagy of gastric cancer cells and its mechanism.Research methods According to the first part of the experiment,THSG on gastric cancer MGC803 and MGC7901 cell IC50 select editing concentration of gastric cancer cells,and then flow apoptosis detection and electroscopy analysis of the apoptosis and autophagy phenomenon of two gastric cancer cells q PCR detects apoptosis-related genes Bax,Caspase-3,PARP,Caspase-7,Bcl-2,and autophagy-related genes LCB3,Beclin-2,ATG3 The m RNA expression level of ATG7;Western blot detects protein expression levels of two gastric cancer cell apoptosis-associated genes Clevead-Caspase-3,Clevead-PARP,Clevead-Caspase-7 autophagy-7 autophagy-related genes LC3 AAB,ATG3,ATG7,and ATG5.Results After gastric cancer MGC803 and MGC7901 cells were treated by THSG,flow apoptosis analysis and detection showed that the proportion of apoptosis cells in two gastric cancer cells increased significantly with the increase of drug concentration compared with the control group.The results of the death and autophagy phenomenon.q PCR showed that the two apoptosis-related genes of gastric cancer cells were Significantly increased on average(P < 0.05)in the two apoptosis-related genes of gastric cancer cells compared to the control group.Western blot results showed that two treated gastric cancer cell apoptosis-related genes Clevead-Caspase-3,Clevead-PARP,Clevead-Caspase-7 autophagy-related genes LC3 AAB,ATG3,ATG7,ATG5 proteins Expression of horizontal apoptosis-related genes Clevead-Caspase-3,Clevead-PARP,Clevead-Caspase-7 autophagy-related genes LC3 AAB,ATG3,ATG7,ATG5 protein expression significantly increased on average(P < 0.05).Conclusion THSG induces apoptosis and autophagy in gastric cancer cells.3 Study on the Damage Effect of THSG on Liver Tissues and Hepatocytes Objective To investigate the effects of THSG on liver and liver cells.Methods 48 mice were randomly divided into distyrene glycosides(Group A)and control group(Group B),each group of 10.Group A according to the dose of 1g/kg,Group B to give the corresponding amount of physiological saline,continuous gastric feeding of rats for 28 days.During the process of administration,the general state of rats was observed.After the last administration,the detection of serum liver function-related biochemical indicators,hestaining observation liver tissue pathomorphology changes,western blot detection liver tissue apoptosis-related protein expression;the use of human normal liver cell line LO2 cells,such as animal experiments.Group A used 7 different concentrations to culture 24 h and 48 h,Group D added physiological saline culture at the same time.CCK8 was used to observe cell proliferation,and PCR was used to detect the expression of apoptotic-related factorm m RNA.Results After 28 days of feeding,there was no significant difference in weight,intake and control ratio in A rats(P > 0.05),liver pathology in A rats was normal,liver biochemical indicators were not statistically different(P-0.05),and apoptosis expression did not change(P > 0.05);Group A showed no statistical difference in the normal liver cell survival rate of LO2 under distyrene sideside treatment(P > 0.05),There was no statistical difference in the expression of hepatocellular apoptosis factor m RNA(P > 0.05).Conclusion THSG has no damage to liver tissues and hepatocytes.