Association Of The DNAH11 Rs2214326 Single Nucleotide Polymoprhism And The Risks Of Coronary Heart Disease And Ischemic Stroke

Objective:At present,it is believed that atherosclerosis is the common cause and pathogenesis of coronary heart disease(CHD)and ischemic stroke(IS),and abnormal blood lipid metabolism is the main risk factor for atherosclerosis.At the same time,with the progress of genetics research,the influence of genetic susceptibility on ischemic cardio-cerebrovascular diseases has gained a deeper understanding.A genome-wide association study(GWAS)found that dynein axonemal heavy polypeptide 11(DNAH11)single nucleotide polymorphism(SNP)is associated with human blood lipid levels and ischemic cardiovascular and cerebrovascular diseases.This article aims to explore the effect of DNAH11 rs2214326 SNP and blood lipid levels in Guangxi Han population on CHD and IS.Methods:This study was based on a case-control study of the Han population in the Guangxi Zhuang Autonomous Region.1,603 subjects were collected in this study.There was a normal control group including 553 normal subjects,and two case groups of CHD(n=536)and IS(n=514)patients.Collect relevant information and clinical data of the research subjects in the form of questionnaires,and conduct a comprehensive physical examination.The subjects were tested for blood lipids and whole blood genome deoxyribonucleic acid(DNA)extraction.The genotypes of the DNAH11rs2214326 SNP were detected using the Snapshot technology.Results:1.The genotype and allele frequencies of the DNAH11 rs2214326SNP were significantly different between the control and case(CHD and IS)groups(P<0.05).Compared with non-G allele carriers,DNAH11 rs2214326 G allele carriers have increased risk of CHD and IS(CHD risk:GA vs AA:OR=1.326,95%CI=1.002-1.756,P=0.049;GG vs AA:OR=3.046,95%CI=1.465-6.331,P=0.003;IS risk:GA vs AA:OR=1.414,95%CI=1.018-1.850,P=0.011;GG vs AA:OR=2.272,95%CI=1.088-4.748,P=0.019).2、Hierarchical regression analysis shows that carriers of the G allele have an increased risk of CHD in the following subgroups:Male(OR=1.576,95%CI=1.130-2.198,P=0.007),Age≤60 years old(OR=1.557,95%CI=1.091-2.223P=0.015),BMI≤24 kg/m~2(OR=1.741,95%CI=1.237-2.451,P=0.001).Similarly,the risk of IS increased mainly in the following subgroups:Male(OR=1.657,95%CI=1.203-2.283,P=0.002),Age>60 years old(OR=1.512,95%CI=1.047-2.183,P=0.027),BMI≤24 kg/m~2(OR=1.464,95%CI=1.042-2.055,P=0.028).Smoking(OR=1.795,95%CI=1.175-2.743,P=0.007),non-drinking(OR=1.491,95%CI=1.058-2.099,P=0.022),hypertension(OR=1.800,95%CI=1.174-2.762,P=0.007).2.The DNAH11rs2214326 SNP was not significantly associated with serum lipid levels in the control group.Conclusion:1.The risk of CHD and IS in carriers of DNAH11 rs2214326G allele is increased in Guangxi Han population.2.DNAH11 rs2214326 SNP interacts with environmental factors(age,gender,smoking,BMI value,and hypertension),affecting the risk of CHD and IS in Han residents in Guangxi.