Neuroprotective Effect And Mechanism Of Wogonoside On Learning And Memory Impaired Mice

ObjectiveWe want to study the neuroprotective effect and mechanism of wogonoside on learning and memory impairment model mice,and the therapeutic effect of wogonoside on hydrogen peroxide-induced SH-SY5Y cell injury model.MethodsIn vivo,a scopolamine-induced learning and memory impairment mouse model was established.The classical Morris water maze test and new object recognition test were used to observe the improvement of spatial learning and memory ability of mice after administration of wogonoside.Enzyme-linked immunosorbent assay(ELISA)kit was used to detect oxidative stress-related indicators in mouse cerebral cortex and hippocampus,and Western blot was used to detect apoptotic proteins and neuroprotective proteins in mouse brain tissue.In vitro experiments,a hydrogen peroxide-induced injury model of human neuroblastoma cells(SH-SY5Y)was established and treated with wogonoside.The morphology of the cells was observed under the microscope.The cell activity was detected by CCK8 kit,and then the content of antioxidant substances in the cells was detected by enzyme-linked immunosorbent assay(ELISA).The expression levels of apoptosis-related proteins in human neuroblastoma cells were detected by Western blotting.ResultsIn vivo studies showed that wogonoside improved the oxidative damage status of the brain,reduced cell apoptosis and improved the memory and learning ability of mice in scopolamine-induced learning and memory impairment model mice.In vitro studies showed that wogonoside reduced cell apoptosis,improved cell viability,and increased the content of antioxidants in the model of oxidative damage induced by hydrogen peroxide in human neuroblastoma cells.ConclusionAs a kind of f lavonoids extracted from Scutellaria baicalensis,wogonoside can improve the learning and memory ability of mice with learning and memory impairment model,while playing a protective role on nerve cells.It has shown a significant role in antioxidant activity and can be further studied as a potential drug for the treatment of cognitive impairment.