Research Of Microfluidic Chip For Antitumor Drug Development

Cancer has become an important killer of human health nowadays,which poses a great threat to people’s life and work.Since the development of platinum drug treatment strategy has made tremendous progress,there is still demand on rapid,efficient,low-cost analytical tools on the optimization of mechanism research and treatment plan.Based on the rapid development and application of microfluidics,microchip provides excellent research platform in the field of medical biochemistry with the advantages of miniaturization,high throughput and low consumption.The study of drug delivery in the chip provides significance for subsequent drug discovery and drug screening.This paper focused on the drug delivery process of platinum drugs targeting cancer cells,we developed a variety of types of microfluidic chip on the basis of hydrodynamic,through the cultured Hela cells as a representative of cancer cells,we obtained the best structure and optimal experimental parameters,on this basis,the different concentrations of platinum drug on the cancer cells were verified.In the design of chip structure,six types of chip structures were obtained by material and technological process selection.Hela cells were selected as the source materials of cancer cells were provided for the experiment through cell culture and extraction,the capture characteristics of six kinds of configuration chips were evaluated and screened to obtain the applicable structure to this study.In the study of experimental conditions,through setting different driving speed(0.1μl/s～0.5μl/s)to explore the law of driving speed on the cell capture efficiency,the cells steady-state time and sample consumption,to obtain the best experimental parameters.On the basis of the optimal structure and parameters,the results of different concentrations(0 μg/ml～100 μg/ml)of platinum drugs on the cells were established,the survival rates of the cells were obtained,and the drug dosing functions of the chips were verified.Through the chip function and cell dosing experiments,we obtained the best structure for this study(double-slit:2.0)and the best experimental parameters(v=0.2 μl/s),in this condition,we achieved high cell capture efficiency in microfluidic chip,through the drug experiments to verify the feasibility of the drug delivery function in this study,the establishment of unit model in microfluidic platform provide research value for the optimization of chip integration and platinum drug development.