Studies On The Chemical Constituents And Biological Activity Of Pogostemon Cablin And Sophora Flavescens

This thesis consists of five chapters.The first chapter reviews the research progress of the chemical constituents and biological activities of Pogostemon cablin（Blanco）Benth and Sophora flavescens.The second chapter discusses the study on the chemical composition and biological activity of the dried rhizome of Pogostemon cablin.The third chapter discusses the chemical composition and biological activity of the dried root of Sophora flavescens.The fourth chapter mainly discusses the possible mechanism of anti-influenza virus effect of traditional Chinese medicine Pogostemon cablin by using network pharmacology.The fifth chapter is a summary of the work done in this paper.Pogostemon cablin is a traditional well-known medicine in China,which has a pungent and mild temperature;it has the effect of releasing heatstroke,and it is used for dampness and turbidity,heat and wet syndrome,fever and fatigue,chest tightness,abdominal pain,vomiting and diarrhea.In this experiment,sephadex LH20,silica gel,reversed-phase chromatography（RP-18）and high performance liquid chromatography（HPLC）were used to separate and identify 15 compounds from the dried rhizome of Pogostemon cablin,including 6 guaiacolanes,2 patchouli alcohol type sesquiterpenes,1 chain sesquiterpenes,3 flavonoids,and 3 other types of compounds.Two new compounds were identified.The anti-influenza virus activity of Pogostemon cablin all polar segments and some compounds was detected by biological methods.The results showed that the ethyl acetate phase of Pogostemon cablin had a significant inhibitory effect on influenza virus WSN with IC₅₀of 127.25μg/ml,and compound 7 had a certain anti-influenza virus effect on PR8,with IC₅₀of 49.56μM.In addition,3 and 7 have a significant inhibitory effect on the Tamiflu-resistant H1N1 virus strain A/Puerto Rico/8/1934,with EC₅₀ of 0.015 and 0.73μg/ml.In addition,we also analyzed the anti-influenza effect of Pogostemon cablin through network pharmacology.Through analysis,it is found that the anti-influenza effect of Pogostemon cablin is mainly guaiac type and patchouli alcohol type sesquiterpene,and may affect the MAPK signaling pathway and PI3K-Akt signaling pathway to regulate cell apoptosis,and then influencing the replication process of influenza virus in the body.But the specific mechanism still needs further confirmation by biological experiments.Sophora flavescens has a medicinal history of more than 2,000 years in China.It has been used as a medicine in the roots.It has the functions of clearing away heat and dampness,killing insects and diuresis,and is mainly used to treat heat d fevers,dysentery,jaundice,leukorrhea,swelling,pain and so on.In this experiment,through sephadex LH20,silica gel,reversed-phase chromatography（RP-18）and high performance liquid chromatography（HPLC）,7 compounds,including 6 lignin compounds and 1 other types of compounds,were isolated and identified from the dried rhizome of Sophora flavescens,and evaluated their anti-inflammatory activity in vitro,and the results showed that all compounds had no obvious activity.