Studies On The Chemical Constituents And Bioactivities Of Scutellaria Barbata

The malignant tumors mortality and morbidity worldwide were rising year by year,which has seriously threatened human life safety and social development,and become a major public health issue of global concern.Chemotherapy plays the most important role in treating tumors,in addition to surgery.However,with the continuous intake of chemotherapy drugs,the tumor cell appeared multi-drug resistance,so that the effect of chemotherapy drugs reduced or completely lost.Finding novel anti-tumor drugs and solving the multi-drug resistance of tumor cells are urgent problems to be solved in the tumor treatment.Tumor multi-drug resistance reversal agents can reverse the drug resistance of tumor cells,restore the sensitivity of tumor cells to chemotherapy drugs,and improve the role of chemotherapy drugs.However,the existing tumor multidrug resistance reversal agents most only target single drug resistance mechanism and target,with lowly reversal efficiency and highly side effects.Scutellaria barbata D.Don is the dry whole grass of the genus Astragalus,which mainly contains flavonoids,neo-clerodane diterpenes,polysaccharides,triterpenes,sterols,volatile oil and trace elements.Among them,neo-clerodane diterpenes have various bioactivities,such as anti-inflammatory,anti-viral and anti-tumor,reverse tumor multidrug resistance,antibacterial activity.This study used modern spectroscopy methods such as silica gel normal phase chromatography,reverse phase material chromatography,gel chromatography,HPLC and NMR,HRMS,UV,IR.Finally,31 compounds(4 new compounds)were isolated from the chloroform extraction part,in which 24 coumpunds were neo-clerodane diterpenoids,7 other types compounds.The results of pharmacological experiments showed that neo-clerodane diterpenoid compounds 10,15,16,22,and 24 were able to reverse multi-drug resistance of Hep G2/Adr cells to adriamycin(Reversal Factor: 15.72-22.51).In addition,some neo-clerodane diterpenoids were tested for the inhibition of NO production in vitro,but none displayed the inhibition capability.