| Kidney cancer is one of the most common cancers,accounting for about 3% of the total number of cancers.Because of its insidious onset,symptoms are already in the middle and advanced stages,so it progresses rapidly and is highly malignant.With the deterioration of the global environment,the increase in living pressure,and the formation of bad habits(such as smoking and staying up late),the incidence and mortality of kidney cancer continue to rise worldwide.Although clinical progress has been made in the treatment of kidney cancer in recent years,including a variety of target drugs approved by the FDA for the treatment of patients with renal cancer,adverse prognosis and adverse reactions after treatment are still a huge obstacle to the treatment effect..Therefore,exploring the pathogenesis of renal cancer in molecular biology and molecular genetics,and discovering new diagnosis and treatment methods based on this can provide new ideas and clues for the treatment of renal cancer.PHF1 not only has the characteristics of PcG protein,it is a histone apparent modification factor,but also contains two PHD zinc finger domains,which may play the role of a transcription factor.Proteins with high homology to PHF1(such as EZH1 and MTF2)have important roles in tumorigenesis and development.Therefore,PHF1 may also play an important role in tumorigenesis,cycle regulation,aging,DNA damage repair,and apoptosis.Role.Our previous research found that PHF1 can exert tumor suppressive effects through the tumor suppressor p53.However,PHF1 is expressed differently in different tumor types or different stages of tumors,and may regulate tumor cell fate through different downstream in different circumstances,and has a complex regulatory mechanism.We also found that PHF1 has a positive regulatory effect on c-MYC in renal cancer cells.The coding gene of c-MYC is an oncogene,which can promote tumorigenesis and development by promoting cell cycle and resisting apoptosis.Our research found that: Real-time q PCR verified that PHF1 positively regulates MYC transcription in renal cancer cell lines;analysis based on the UALCAN database found that both PHF1 and MYC were overexpressed in renal cancer tissue samples,and that they had a positive correlation before;Western Blot verified that PHF1 is positively regulating MYC at the protein level in renal cancer cell lines;Co-ip confirmed that protein interaction exists between PHF1 and MYC.Our findings may provide data to support the molecular mechanism of renal cancer and the discovery of new targets for treatment.p53 is a stable tumor suppressor.However,mutations occur in more than 50% of tumors.Mutated p53 cannot continue to perform the role of tumor suppressor and may promote cancer.Therefore,p53 is an important target for tumor therapy,and the reverse mutation of p53 to wild-type function is also the main idea based on p53 drug development.This study will use fluorescence resonance energy transfer technology to construct probes that can observe the effect of drugs on the molecular structure of p53 in real time,and provide a method and platform for drug screening based on changes in p53 structure. |
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