| Background: Esophageal Cancer(EC)is a malignant tumor with a high morbidity and mortality rate.It accounts for more than 400,000 new diagnoses worldwide each year.The pathogenesis of esophageal cancer is very complicated,which is generally the result of multiple factors.Since the causal relationship between H.pylori and gastric cancer was determined,the relationship between specific bacterial infection and corresponding tumor occurrence and development has become one of the hot spots in tumor research in recent years.Porphyromonas gingivalis(P.gingivalis)is traditionally considered as the main pathogen of periodontitis,but recent studies have found that its infection is associated with a variety of systemic diseases,including atherosclerosis,diabetes and oral cancer.In 2012,Ahn et al.found that P.gingivalis content was correlated with the risk of oral cancer,and was positively correlated with the mortality of oral gastrointestinal cancer.They believed that P.gingivalis infection could increase the mortality of tumor patients,providing strong evidence for the study on the relationship between P.gingivalis and tumor.The latest studies by gao shegan et al of henan university of science and technology showed that the detection rate of P.gingivalis in esophageal squamous cell carcinoma was 61%,and the detection rate in adjacent tissues was 15%,indicating that P.gingivalis may be one of the important bacterial factors inducing esophageal squamous cell carcinoma and is closely related to the occurrence,development and prognosis of esophageal squamous cell carcinoma(ESCC).Objective: To analyze the infection status of P.gingivalis in oral squamous cell carcinoma and esophageal squamous cell carcinoma of different thoracic segments.The relationship between P.gingivalis infection and the clinicopathological characteristics of patients with upper,middle and lower esophageal thoracic ESCC was analyzed.Methods:1.Retrospective selection of postoperative pathological samples and relevant clinicopathological data of 50 cases(R0 resection of t1-3nxm0)of esophageal suprathoracic,middle and lower esophageal squamous cell carcinoma and oral squamous cell carcinoma in our hospital.The exclusion criteria were: neoadjuvant therapy(radiotherapy,chemotherapy,radiotherapy and chemotherapy)before surgery;Antiinflammatory treatment was performed 1 week before surgery.2.Immunohistochemistry was used to detect the expression of P.gingivalis specific protein in oral squamous cell carcinoma and various thoracic esophageal squamous cell carcinoma tissues,and to analyze its correlation with clinicopathological characteristics and prognosis.3.Statistical analysis: SPSS23.0 software was used for statistical analysis of all statistical data,and P<0.05 was considered as the statistical difference with statistical significance.Results:1.Immunohistochemistry showed that P.gingivalis was mainly implanted in the cytoplasm of oral squamous cell carcinoma and esophageal squamous cell carcinoma,showing brown-yellow granules.2.In the oral squamous cell carcinoma group and the upper,middle and lower esophageal squamous cell carcinoma group,P.gingivalis negative group was generally superior to the positive group in staging and pretreatment,suggesting that P.gingivalis may be involved in the occurrence and development of oral squamous cell carcinoma and esophageal squamous cell carcinoma.3.Through immunohistochemical detection,P.gingivalis has a certain detection rate in oral and upper,middle and lower esophageal squamous cell carcinoma tissues.Among the 50 patients with oral squamous cell carcinoma,27(54.0%)were P.gingivalis positive.Among the 50 patients with upper esophageal squamous cell carcinoma,24(48.0%)were P.gingivalis positive.Among 57 cases of middle esophageal squamous cell carcinoma,24(42.1%)were P.gingivalis positive.Among the 50 patients with lower esophageal squamous cell carcinoma,20(40.0%)were P.gingivalis positive.4.P.gingivalis was significantly correlated with infiltration depth(P=0.012),lymph node metastasis(P=0.011),clinical stage(P=0.001)and survival(P=0.03)in oral squamous cell carcinoma patients,and the difference was statistically significant.5.P.gingivalis was significantly correlated with alcohol consumption(P=0.049),infiltration depth(P=0.026),lymph node metastasis(P=0.001),clinical stage(P=0.003)and survival(P=0.039)in patients with esophageal upper thoracic squamous cell carcinoma,and the difference was statistically significant.6.P.gingivalis was significantly correlated with age(P=0.011),infiltration depth(P=0.001)and lymph node metastasis(P=0.001),and the difference was statistically significant.Since all patients in this group were newly diagnosed with esophageal cancer within 18 years,the number of deaths was relatively small,only 1person.Survival analysis was meaningless,and the survival curve was analyzed after3-5 years of follow-up.7.P.gingivalis was significantly correlated with gender(P=0.033),alcohol consumption(P=0.005),lymph node metastasis(P=0.002),clinical stage(P=0.012),and survival(P=0.001)in patients with esophageal carcinoma of the lower thoracic segment,with statistically significant differences.Conclusion:1.The P.gingivalis positive rate of oral squamous cell carcinoma group was significantly higher than that of the upper,middle and lower esophageal squamous cell carcinoma group,and the positive rate decreased successively from top to bottom,with statistically significant difference(P<0.05).2.P.gingivalis had a significant correlation with the clinicopathological characteristics of oral squamous cell carcinoma and esophageal squamous cell carcinoma,and the difference was statistically significant,and the correlation showed a downward trend from the oral cavity to the lower thoracic segment of the esophagus.3.The study showed that the survival status of patients in the P.gingivalis negative group was generally better than that in the P.gingivalis positive group,suggesting that P.gingivalis infection may be one of the biomarkers and risk factors for oral squamous cell carcinoma and esophageal squamous cell carcinoma. |
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