| OBJECTIVE:This research will explore:1)The origin,type and anatomical features of the thoracic segment of aortic perivascular adipose tissue(PVAT);2)Wether PVAT may influence the aortic vascular function.METHODS:Thoracic segment of aortic PVAT were collected from mice with different satge:oil red staining,H&E staining and UCP1 Immunohistochemistry was performed to determine the development and cell morphology and composition of thoracic segment of aortic PVAT.Lineage tracing mice were constructed to explore the origin of thoracic segment of aortic PVAT.RNA-seq was perfomed to analyze the differences in transcriptional profiles.Adipose-Derived Mesenchymal Stem Cells(ADSCs)markers and inflammatory cell markers were quantified by flow cytometry.Aortic ring assay was performed to compare the effects of adipose tissue in different parts of PVAT on vasomotor properties.RESULTS:We found that thoracic segment of aortic PVAT was consisted of thoracic aortic perivascular adipose tissue(T-PVAT)and periaortic arch adipose tissue(PAAT),each of them has its own unique anatomical structure,origin and development process.Lineage tracking studies showed that T-PVAT adipocytes were mainly derived from SM22α~+and Myf5~+adipocytes.PAAT was mainly developed from SM22α~+,Myf5~+adipocytes and NCCs-derived precursor cells.There were special physiological and pathological functions in different parts and different lineages of PVAT.CONCLUSION:Our studies illustrate the distinctive anatomical features and developmental programs of PVAT,and reveal several lineage cells are the contributers of PVAT.Most of PAAT are origin from NCCs which is a progenitor derived from ectoderm.This is the first reported that NCCs is a brown adipocyte progenitor other than white progenitor.Our studies provide solid evidences that the origin and development of PVAT are much more complex than previously anticipated,and adipocytes derived from different origins and districts have distinct transcriptional profile. |
PDF Full Text Request