HPLC-ELSD Determination Of Main Active Components In Three Kinds Of Chinese Traditional Medicinal Crops With Homology Of Medicine And Food

With the increasing demand for health care and health preservation,the application of "medicine and food homology" raw materials in food,medicine and health food is more and more extensive.The quality control level of these "medicine and food homology" raw materials in medicine,health products,especially in food production industry has become an important topic.In this paper,the economic,rapid and accurate methods of HPLC-ELSD(High performance liquid chromatography-evaporative light scattering detection)for detecting the main active components in astragalus,platycodon grandiflorum and yam are established.They can be used as a reference for the quality control of raw materials in platycodon grandiflorum,astragalus and yam related medicine,health products and food industry.The specific contents and conclusions of this paper are as follows:(1)A HPLC-ELSD method for the determination of platycodon D in platycodon grandiflorum is established and validated.The standard curve method is used for quantitative analysis of platycodon D,and the linear regression equation is Y=1.4309X+3.4918,r=0.9996.According to the standard curve of platycodon D,it can be seen that in the range of 0.95 μg~9.5 μg,there is a good linear relationship between the target injection mass and the corresponding peak area.The detection limit of platycodon D is 0.08 μg,the quantitative limit is 0.24 μg,the average RSD of the standard product peak area of platycodon D is 0.66%,the RSD of platycodon D in the parallel sample of platycodon sample solution(n=6)is 2.07%,the RSD of the peak area of platycodon D in the same platycodon sample solution within 24 hours is 0.93%,the content of platycodon D in the selected platycodon sample is 0.19%,and the average recovery rate(n=9)is 100.19%,RSD value is 2.10%.The results show that the sensitivity,precision,reproducibility and stability of the method are good,the results are accurate and reliable,and meet the requirements of methodology validation.(2)A HPLC-ELSD method for the determination of astragaloside in astragalus is established and validated.The quantitative analysis of astragaloside is carried out byusing the standard curve method.The linear regression equation is Y=1.3578X+3.6628,r=0.9995.According to the standard curve of astragaloside,it can be seen that in the range of 0.88 μg~8.8 μg,there is a good linear relationship between the target injection mass and the corresponding peak area.The detection limit of astragaloside is 0.07 μg,the quantitative limit is 0.21 μg,the average RSD of astragaloside peak area is 0.68%,the RSD of astragaloside content in the parallel sample of astragalus sample solution(n=6)is2.00%,the RSD of astragaloside peak area in the same astragalus sample solution within24 hours is 0.97%,the content of astragaloside in the selected astragalus sample is 0.16%,and the average recovery rate(n=9)is 99.92%,RSD value is 2.14%.The results show that the sensitivity,precision,reproducibility and stability of the method are good,the results are accurate and reliable,and meet the requirements of methodology validation.(3)A HPLC-ELSD method for the determination of dioscin in yam is established and verified.The quantitative analysis of dioscin is carried out by the standard curve method.The linear regression equation is Y=1.3415X+3.8379,r=0.9997.According to the standard curve of dioscin,it can be seen that in the range of 0.56 μg~5.6 μg,there is a good linear relationship between the target injection mass and the corresponding peak area.The detection limit of dioscin is 0.05 μg,the quantitative limit is 0.15 μg,the RSD of dioscin standard peak area is 0.65%,the RSD of dioscin content in the parallel sample solution(n=6)is 1.69%,the RSD of dioscin peak area in the same yam sample solution within 24 hours is 0.98%,the content of dioscin in the selected yam sample is 0.0357%,and the average recovery rate(n=9)is 99.62%,RSD value is 1.67%.The results show that the sensitivity,precision,reproducibility and stability of the method are good,the results are accurate and reliable,and meet the requirements of methodology validation.