Preparation And Properties Of Hyaluronic Acid-based Composite Gel Materials

At present,the treatment of bone defects is quite difficult,especially the treatment of cartilage defects.Commonly used treatment with bone autograft and allograft,but due to problems such as source,price,immune and bone graft method can completely meet the requirements of clinical treatment.The combination of biomaterials and tissue engineering technology has become a new direction of medical research.Hydrogel is a hydrophilic polymer by chemical crosslinking or by intramolecular and intermolecular physical together the three-dimensional network of gravity.In research of bone tissue engineering cartilage material,with biocompatibility,biodegradation absorbability,plasticity and preparation of hydrogels is becoming a hot spot of research.Although various biological materials can form hydrogels,hyaluronic acid and its derivatives are the most widely used.Hyaluronic acid due to its good viscoelastic,biocompatible and biodegradable properties of biomedical field widely used.Hyaluronic acid hydrogel has the advantages of promoting cell adhesion and proliferation.They also show sufficient physical and chemical properties to support the environment in which the cells live and have a good advantage in the application of bone tissue engineering.In this paper,the hydrogels were prepared based on hyaluronic acid/methyl cellulose,and their physical and chemical properties and biocompatibility were studied.(1)First,hyaluronic acid(good biocompatibility)and polyethylene glycol succinimidyl succinate are cross-linked by covalent reaction to form a hyaluronic acid derivative(HA-PEG)with amide bonds.Then the temperature-sensitive methyl cellulose is used as the base material,and the system ratio of each component is controlled,and mineralized collagen is doped in the system to prepare a temperature-sensitive hyaluronic acid-based hydrogel.The hyaluronic acid-mineralized collagen hydrogel formed based on the amide bond can realize rapid sol-gel transition under physiological environment.The rheological characterization results show that the hydrogel retains the unique rheological properties of hyaluronic acid.Scanning electron microscopy(SEM)shows that the hydrogel has a porous interconnected and stable three-dimensional network structure,which is conducive to cell adsorption and proliferation.The results of protein concentration characterization and alkaline phosphatase staining show that the cells grow well,which proves that hydrogels have good cellular compatibility and can be used in the field of bone tissue engineering.(2)A new type of hyaluronic acid/graphene oxide composite hydrogel was prepared by doping the hyaluronic acid-based hydrogel with graphene oxide,a nanomaterial with good mechanical properties.On its rheological properties,scanning electron microscope,the swelling performance and to study the cytotoxicity,results show that the hyaluronic acid/graphene oxide composite hydrogel has good viscoelastic,no cytotoxicity,etc.(3)Graphene is a carbon-based material with a two-dimensional flat carbon atom structure,which can promote cell attachment and proliferation,and can also induce and enhance osteoblast differentiation.By combining graphene and transparent acid hydrogels,form a new composite hydrogel.Tests on its physical and chemical properties and cytotoxicity show that the composite hydrogel has excellent properties and has broad application prospects in tissue engineering...