The Burn Healing Effect Of Snail Mucus And The Extraction,Purification And Characterization Of Its Effective Components

White jade snail was known as Achatina fulica,the protection,moisturizing,antibacterial,repairing and other properties of snail mucus make it have potential application value.In Chinese folk medicine,snail mucus had been used for burn management,but the effective ingredients that played a role in burns were not known,the mechanism of repairing lacked systematic research.Exploring the effective ingredients and characterizing their structure are conducive to the interpretation of the structure-activity relationship,and the results are also of great significance to the upgrading of the snail industry.In this study,the white jade snail mucus was extracted,the physical and chemical properties of snail mucus were tested.The burn mouse model was established to evaluate the effect of snail mucus on burn healing.The effective ingredients of wound healing were separated,purified,and characterized.The main research was as follows:(1)Extraction and physicochemical properties of snail mucusThe different extraction methods of snail mucus were compared,the extraction method was breaking the snail shell,according to the material-to-liquid ratio of 1:2,the ultrapure water was added to extraction for 2 h.The results of snail mucus composition,physical and chemical properties showed that snail mucus was mainly composed of protein and polysaccharides,and the snail mucus had good adhesion.The protein content,total sugar content and adhesion force of snail mucus in different environment were compared.The results showed that the powder state of snail mucus was more stable than the solution state,and-20℃ was more conducive to storage than the normal temperature environment.The DPPH scavenging activity and total reducing power showed that the antioxidant activity in vitro of snail mucus are good.(2)The effect of snail mucus on wound healing in burned miceThe effect of snail mucus on wound healing in burned mice was explored.The results showed that snail mucus could effectively promote wound healing in burned mice.The early wound closure was related to the antioxidant and anti-inflammatory activities of snail mucus.At the same time,snail mucus could promote the secretion of epidermal growth factor and accelerate wound healing.(3)Separation,purification and structural analysis of snail mucusSnail mucus was purified by DEAE Sepharose FF ion exchange chromatography and Sephacryl S-300 gel chromatography,a glycoprotein of SM1 S was obtained.The results of cell migration experiments showed that SM1 S could accelerate the scratch closure of fibroblasts.The structure of SM1 S was characterized by high performance liquid chromatography,electron microscopy,nuclear magnetic resonance and electron microscopy.The results showed that SM1 S contained no impurities such as nucleic acid and protein,the content of glucuronic acid was 5.61%.The main component of SM1 S was glycoprotein with a molecular weight of 37727 Da(83.23%),SM1 S was composed of mannose,glucuronic acid,glucose,galactose,xylose,arabinose,fucose,and its molar ratio was 0.012:0.004:0.97:0.007:0.001:0.003:0.002.The results of infrared spectrum showed that SM1 S contained O-H,C-H,C=O bonds and amido bond.Periodic acid oxidation and nuclear magnetic resonance analysis showed that SM1 S contained 1,6-glycosidic bonds,possibly 1,4-or 1,2-glycosidic bonds.The results of electron microscopy showed SM1 S was flake-like and the surface of SM1 S was uniform.This study showed that snail mucus could promote wound healing in burned mice,and the effective ingredient was glycoprotein SM1 S,which provided a theoretical basis for the development of snail mucus products.