| ObjectiveTo explore the protective effect of sodium ferulate on nerve injury in AD rats.To study whether sodium ferulate can protect the nerve injury of AD rats through AMPK-mTOR pathway.MethodsTwo-month-old SD rats were adaptively reared for one week and then injected Aβ25-35into the lateral ventricle to construct an AD model,and were given sodium ferulate solution 100mg/kg and 150mg/kg respectively and 4 week.Grouped into the control group,AD model group,AD+SF100mg/kg group,AD+SF150mg/kg group.Record the general condition of the rat,weight,activity,and stool status.Use open field experiments and water mazes to detect changes in behavior.He staining was used to observe the changes of neurons and Nissl staining was used to observe the condition of vertebral cells.Western blot experiments to measure p-AMPK、p-mTOR、P70S6K、ATG protein and related proteins tau、IBA1、GFAP proteins.ResultsThe number of neurons in the mouse brain is reduced,the cell arrangement is loose and disordered,and the cells often appear broken or wrinkled Condensed morphological changes,administration of sodium ferulate（100mg/kg or 150mg/kg）can signifitcantly improve the nerve damage in AD model rats;Nissl staining results showed that Nissl bodies in the brain of AD group rats decreased,the volume decreased,and the color became lighter.Sodium verulate can significantly improve the destructive phenomenon of Nissl body;Western blot results showed that the expression levels of p-AMPK、ATG、Tau、IBA1 and GFAP protein in the brain tissue of AD group increased,and the expression levels of p-mTOR、P70S6K protein decreased.Compared with the model AD group,the expression levels of p-AMPK、ATG、Tau、IBA1 and GFAP protein in the brain tissue of AD+SF100mg/kg group decreased,and the expression levels of p-mTOR、P70S6K protein increased.ConclusionsTo some extent,Sodium ferulate can improve the nerve injury of AD rats;Sodium ferulate on the nerve damage of AD may be related with the AMPK-mTOR signal pathway. |
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