Correlation Of EIF2S2 Expression With PET/CT Metabolic Parameters In Colorectal Cancer

ObjectiveTo explore the correlation between EIF2S2 expression and PET/CT metabolic parameters and aerobic glycolysis in colorectal cancer.MethodsThe expression of EIF2S2 in colorectal cancer and its relationship with clinical features were obtained from the databases UALCAN and ONCOMINE.Analysis of the frequency of EIF2S2 gene alterations through the cBio Portal database.The expression of EIF2S2 in relation to GLUT1 was obtained by immunohistochemical staining of 42 colorectal cancer patients who had undergone PET/CT(no radiotherapy treatment before surgery).The correlation between EIF2S2 and glycolytic-related gene sets was obtained from the GEPIA database.Colon cancer cells SW480 were cultured and transfected with EIF2S2-interfering RNA to detect lactate production and mRNA levels of selected glycolysis-related genes.ResultsThe database showed that EIF2S2 was highly expressed in colorectal cancer tissues compared with the paracancerous,and that the EIF2S2 gene had a certain frequency of mutation and amplification in colorectal cancer tissues.The high expression was verified by immunohistochemistry and the corresponding colorectal cancer tissues were immunohistochemically stained for GLUT1,and it was concluded that there was a correlation between the expression of EIF2S2 and GLUT1 in colorectal cancer(P < 0.05).The correlation between the expression of EIF2S2 in colorectal cancer patients and clinical characteristics was analyzed,and it was learned that the expression of EIF2S2 correlated with PET/CT parameter SUVmax in colorectal cancer patients,and it was further hypothesized that EIF2S2 correlated with tumor glycolysis in colorectal cancer.The results of the lactate production assay showed that the lactate production of the cells in the experimental group was lower than that of the control group(P < 0.05),while there was no significant difference in the lactate production capacity of the cells between the different control groups.RTq PCR analysis showed that the mRNA expression of SLC2A1、SLC2A3、SLC2A10、HK2、PKM2 and LDHA was reduced in the experimental group compared to the two control groups(P < 0.05),while there was no difference in expression between the different control groups.ConclusionsIn colorectal cancer patients,EIF2S2 is highly expressed in cancerous tissues compared with paraneoplastic tissues.The expression of EIF2S2 affects the glycolytic uptake and aerobic glycolytic pathway products in colorectal cancer,while EIF2S2 has a certain frequency of mutation and amplification in colorectal cancer,so it can be used as a target for gene therapy in colorectal cancer patients.