Study Of Exosomes Derived From Bone Marrow Mesenchymal Stem Cells On The Repair Of Neuronal Oxidative Stress Damage

Objective:This study aims to explore the repair effect of Bone marrow derived mesenchymal stem cells(BMSCs)secretion in the Oxidative Stress(OS)of nerve cells,so as to provide a new theoretical basis and reference for the precise treatment of Central Nervous System(CNS)injury-related diseases.Methods:SH-SY5 Y cell line was selected as the model of nerve cells in vitro,and SH-SY5 Y cells were damaged by 1-methyl-4-phenyl-pyridine ion(MPP+)to establish the model of nerve cells damaged by oxidative stress.Reactive oxygen species(ROS)and cell apoptosis were observed after treated with MPP+ at different concentrations(0.25,0.5,1,2,4 and 8mmol/L)for 24,48 and 72 h.Then,BMSCS-derived secretory agents and BMSCS-derived exosomes were used to treat the injured cell model,respectively.The neuronal survival rate,intracellular ROS content and cell apoptosis were detected to detect the related repair effect.Results:1.Use concentration(0.25,0.5,1,2,4,8 tendency/L)of MPP + added to SH SY5 Y cells build neurons after processing,the oxidative stress model in 24,48,72 hours,respectively,to test the cells,found that SH SY5 Y cells survival rate will be increased with the increase of MPP + dose and time is reduced,and the difference is statistically significant(p<0.05).The intracellular ROS content and cell apoptosis were detected,and it was found that MPP+ could increase intracellular ROS content(p<0.05)and cell apoptosis(p<0.05).2.The supernatant from BMSCs was added to the constructed neuronal oxidative stress model to detect cell survival rate,intracellular ROS content and cell apoptosis.The results showed that cell survival rate was increased(p<0.05),intracellular ROS content was down-regulated(p<0.05),and cell apoptosis was decreased(p<0.05)in the oxidative stress model of neurons treated with the supernatant of BMSCs source.3.BMSCS-derived exosomes were added to the constructed neuronal oxidative stress model to detect cell survival rate,intracellular ROS content and cell apoptosis.The results showed that cell survival rate was increased(p<0.05),intracellular ROS content was down-regulated(p<0.05),and cell apoptosis was decreased(p<0.05)in the oxidative stress model of neurons treated with the supernatant of BMSCs source.Conclusion: 1.MPP+ induces the increase of reactive oxygen species in SH-SY5 Y cells,promotes cell apoptosis and inhibits cell survival.2.Bone marrow mesenchymal stem cell-derived exosomes can inhibit the increase of intracellular reactive oxygen species caused by MPP+,reduce cell apoptosis,and increase the survival rate of SH-SY5 Y cells.