Research On The Effect Of Ginsengdiol Saponins On Cardiac Energy Metabolism In Sepsis Mice

Objective:To explore the therapeutic effect of ginsenediol saponin(PDS)on damaged myocardium in septic mice,on the injured myocardium of septic mice,and to clarify its potential mechanism from the perspective of energy metabolism,so as provide a new perspective for the mechanism of PDS in the treatment of myocardial injury.Methods:1.Forty leaning grade C57BL/6 male mice were divided into 4 groups according to the allocation principle: blank group(control group),model group(LPS group),positive control group(DEX group)and PDS group,with 10 mice in each group.Sepsis model was established by intraperitoneal injection of LPS(10mg/kg)in mice.2.12 h after the establishment of the model,the heart index(heart mass/mouse mass)of the mice was calculated.3.The contents of myocardial creatine kinase(CK)and lactate dehydrogenase(LDH)in mice were determined by ELISA.4.HE staining was used to observe the changes of morphological myofilaments,inflammatory infiltration other structural changes in the myocardium of mice in each group.5.Protein expressions of DUSP1,PFKM,CPT1 A,ACSL4,ATP5A1 and PGC-1α in energy metabolism of myocardial tissues of mice in each group were detected by immunohistochemical detection.Results:1.The results of mouse heart index showed that compared with the blank group,the heart index of the LPS group increased,while the heart index of the PDS group decreased significantly compared with the LPS group(p <0.05).2.The results of HE is staining of mice showed that compared with the blank group,the myocardial structure in the LPS group was seriously damaged,and the damaged myocardial tissue in the PDS group was significantly improved compared with the LPS group(p<0.05).3.ELISA results showed that compared with the blank group,the levels of CK and LDH in the LPS group were increased,and those in the PDS group were decreased compared with the LPS group(p<0.05).4.The immunohistochemical results showed that compared with the blank group,the expression level of DUSP1 protein representing glucose metabolism in LPS group was decreased(p<0.05),and the expression level of PFKM protein was increased(p<0.05).There was no significant difference in the expression of CPT1 A and ACSL4 proteins representing lipid metabolism(p>0.05).The expression levels of ATP5A1 and PGC-1αproteins representing mitochondria were decreased(p<0.05).Compared with LPS group,the expression levels of DUSP1,ATP5A1 and PGC-1α in PDS group were up-regulated,and the expression levels of PFKM protein were down-regulated(p<0.05).Conclusion: Ginsenediol saponins can improve cardiac function and myocardial injury in sepsis to a certain extent,the mechanism of which may be related to the improvement of glucose metabolism and mitochondrial productivity in myocardial energy metabolism.