Study On Preparation And Pharmacodynamics Of Oral Film Containing Effective Ingredient Of Blaps Rynchopetera

Objective:Use the knowledge of network pharmacology to find the possible target and mechanism of drug action on chronic periodontitis.Different batches of effective ingredient Oral membrane of Blaps rynchopetera were prepared,and the stability of the preparation process was evaluated.In vivo experiments were conducted to verify the mechanism of effective monomer film of Blaps rynchopetera on experimental periodontitis in rats.Methods:1.By means of network pharmacology,Gene Cards was used to predict the targets of chronic periodontitis.The effective ingredient B(3,4-dihydroxy phenylacetic acid)was predicted by Swiss Target Prediction.To establish the interaction network between the effective ingredient B target of Blaps rynchopetera and the target protein of chronic periodontitis,to conduct molecular docking,and to search for the possible target and mechanism of the substance’s intervention in periodontitis.2.At different times,three batches of the oral membrane(Ⅰ、Ⅱ、Ⅲ)were prepared according to the same method and auxiliary material ratio.Each batch was divided into blank film agent(01),ingredient B-low dose(02)and ingredient B-high dose drug film agent(03).The appearance,weight difference,thickness,mechanical properties and water content of the three batches of oral membrane were investigated and compared.3.Twenty adult female SD rats were randomly divided into 4 groups according to the random number table,which were model group,positive drug group,ingredient B-low-dose group and ingredient B-high-dose group,with 5 rats in each group.All rats were pressed into the space between the right maxillary first and second molars and second and third molars with 2-0 surgical nonabsorbable sutures,and the sutures were wound around the neck of the second molar.The supernumerary sutures were twice knotted in the palatal proximal middle of the second molar to cut off the supernumerary sutures.Two weeks after the modeling,the rats were anesthetized with isoflurane,and the prepared film was affixed to the palatal side of the second molar of the rats,so that the periodontal tissue of the second molar was completely covered.During the administration,the rats maintained anesthesia for about 5 minutes,and the administration period was two weeks.After administration,the rats were killed,gingival tissue samples were collected and stored in liquid nitrogen,and bilateral maxillary bone samples were collected and stored in formalin solution for micro-CT detection.Results:1.The network pharmacological results predicted that the effective ingredient B of Blaps rynchopetera may act on ARK1B1,MMP-2,MMP-9,CNR1,CNR2 and other proteins,it might interfere the occurrence and development of experimental periodontitis in rats from two aspects of inhibiting bone absorption and anti-inflammatory.2.According to the different time points,the same method and the ratio of material preparation of three batches of the oral membrane,observe its appearance surface level off is smooth,no visible bubbles,and the weight difference between the three batch preparing testing all conform to the stipulations of the 2020 edition of pharmacopoeia.There were no significant differences in thickness,mechanical properties or water content between batches of the same film agent.It can be proved that under the conditions of this process,there is no significant difference between different batches of same oral membrane preparation,that is,the preparation process is relatively stable.3.In vivo experimental results of periodontitis in rats showed that after two weeks of periodontitis administration,the CEJ-ABC distance between the distal first molar and the proximal second molar in effective ingredient B-high dose film group was significantly decreased compared with the model group,and the odd difference was statistically significant.The results of bone microparameter analysis showed that the bone volume fraction(BVF)of alveolar bone between the first and second molars in ingredient B-low dose group was significantly increased compared with that in model group.The cortical bone mineral density(BMD)of alveolar bone of rats in drug administration group was significantly increased compared with model group.Conclusion:1.The preparation process of drug film containing ingredient B of Blaps rynchopetera was relatively stable,and there was no significant difference between different batches of the same film agent.2.Blaps rynchopetera active ingredient B oral membrane has a certain intervention effect on bone resorption of alveolar bone in rats with periodontitis.