Effect Of Surface Modification Of Ultraviolet Photofunctionalized Titanium Dioxide Nanotubes On Osseointegration Of Titanium Implants

Objective : To study the influence of UV photofunctionalized TiO₂ nanotube（UV-TiO₂）surface modification on the osseointegration of dental implants,and analyze the effects of UV-TiO₂ titanium surface modification on the adhesion and proliferation of osteoblasts,and discuss the influence mechanism of UV-TiO₂ Titanium surface modification on the osseointegration of dental implants.Methods:TiO₂ nanotube arrays were formed on the surface of titanium by anodic oxidation method,TiO₂ and nanotube surfaces were photofunctionalized by UV,and changes in titanium surface potential,liquid contact Angle,surface morphology and other indexes were detected.The UV-TiO₂ surface modified titanium implants were implanted into animals and the osseointegration tissue sections of the implants were prepared.The bone binding rate of the implants was observed and calculated to analyze the influence of surface modified UV-TiO₂ on the osseointegration of the titanium implants.Through cell experiments in vitro,a co-culture model of UV-TiO₂ surface modified titanium and osteoblasts was established.Cell staining and MTT were used respectively to detect the changes in the adhesion and proliferation of osteoblasts,and the influence mechanism of UV-TiO₂ surface modified titanium on the osseointegration of titanium implants was discussed.Results: After UV photofunctionalized,there was no significant change in surface appearance compared with the control group,the contact Angle was smaller,and the surface charge of the implant was positive.After 3 months of UV-TiO₂surface-modified titanium implant was implanted into animals,the osseointegration rate of the implant was significantly higher than that of the non-surface-modified group.After co-culture of UV-TiO₂ surface-modified titanium sheet with osteoblasts,the adhesion rate and proliferation rate of osteoblasts were significantly improved compared with those of the non-surface-modified group.Conclusion: The surface modification of TiO₂ can improve the surface performance of titanium,improve the adhesion and proliferation of osteoblasts,and thus promote the osseointegration of implants.