Preparation Of IGF-1R-targeted Ultrasound Microbubbles And Its Application In Prostate Transplantation Tumor Imaging

Objective:Immunochemical technique and Western blot were conducted to analyze the content of IGF-1R protein on the surface of cancer of the prostate gland LNCa P cells.To prepare a targeted ultrasound contrast agent targeting IGF-1R on the surface of LNCa P cells.The naked mice model of manned prostate cancer was established,attempts to explore the ability of IGF-1R targeted ultrasound contrast agent to bind cell targets in vivo.Methods:(1)Androgen-independent LNCa P cells and prostate epithelial RWPE-2cells were cultured.Immunofluorescence method was conducted to explore IGF-1R on the surface of LNCa P cells.The content of IGF-1R on the surface of LNCa P cells and RWPE-2 cells were via Western blot.(2)We generate twenty male immunodeficiency-based mice manned prostate cancer xenograft model,and Immune deficient mice were arbitrarily divided into two groups:the blank comparison group and experimental group.Non-targeted ultrasound contrast agent and IGF-1R antibody targeted ultrasound contrast agent were injected respectively.The tumor was observed and recorded in real time under ultrasound detection and the relevant parameters were analyzed and compared.Results:(1)Western blot assays showed IGF-1R was upregulated on the surface of LNCa P cells.(2)The common ultrasound microbubbles and IGF-1R antibody targeted ultrasound microbubbles were successfully prepared.The two kinds of microbubbles were observed under the microscope.The common microbubbles group refused to dye and did not show green fluorescence,while the targeted microbubbles group showed positive staining and green fluorescence.(3)Through the tail vein of nude mice,the above two kinds of ultrasound microbubbles respectively,the echo of tumor tissue in two groups showed fast in and fast out,and there was no significant difference in the peak time of development intensity between the control group and experiment group(P > 0.05),but there were significant differences in the peak intensity(TP)and area under curve(AUC)of targeted microbubbles(P < 0.05).Conclusion:(1)IGF-1R is expressed in both LNCa P cells and epitheliall prostate cells,and the expression level of IGF-1R in androgen dependent prostate cancer cells is higher than that in normal prostate cells;(2)ultrasound microbubbles can be made by biotin avidin method,and can be targeted with LNCa P cells for ultrasound imaging.