Analysis Of NK Cell Subsets In Peripheral Blood Of Preeclampsia

Objective: With the change of modern living environment and conditions,preeclampsia has become a highly concerned disease.Because of the high incidence of this disease,the physical and mental health of pregnant women and children during pregnancy has formed a greater threat.The onset time of the disease is in the middle and late gestation,with hypertension and proteinuria as the main clinical manifestations,often accompanied by organic and functional damage of most organs in the body;When the symptoms are serious,convulsions,coma,diffuse intravascular coagulation(DIC),cardiovascular and cerebrovascular accidents can occur,and when the symptoms are serious,life can be endangered.Abnormal immune mechanism at the maternal-fetal interface is considered to be an important cause of the onset of preeclampsia.NK cells play an important role in maternal-fetal interface,but its distribution and role have not been fully explored.By analyzing the distribution and status of NK cells in the blood of pregnant women with preeclampsia,the change process of NK cells during pregnancy was explored.Methods: 1.Case selection:A total of 75 pregnant women in the experimental group meeting the diagnosis of preeclampsia were selected according to the inclusion and exclusion criteria,and 75 normal pregnant women were randomly selected as the control group.Inclusion criteria: There was no significant difference in the proportion of lymphocytes and CD3+T cells in peripheral blood between experimental group and control group(P>0.05),the proportion of NK cells in preeclampsia patients was significantly higher than that in normal pregnancy group(P<0.05).3.Exclusion criteria: Complications of chronic hypertension,diabetes,systemic inflammatory diseases,hematological diseases,recent use of glucodermatin hormones or immunosuppressive agents,and other obstetric complications were excluded.4.Flow cytometry was used to detect the changes in the proportion of peripheral blood lymphocytes and NK cell subsets,and the expression of NK cell surface molecules.5.General data were collected for the included women,including age,height,weight,number of pregnancies,gestational week of termination,history of preeclampsia and transfer to the NICU after delivery,score,and birth weight.6.Recording conclusions based on statistical results.Results:1.The general data of the experimental group and the control group were compared.There were no statistically significant differences in age,gestational age,gestational order and birth order between the pregnant women in the experimental group and the control group(P>0.05),and the proportion of NICU conversion in experimental group was higher than that in control group,and the score and birth weight were lower(P&L;0.05).2.There was no statistically significant difference in the proportion of lymphocytes and CD3+T cells in peripheral blood between the experimental group and the control group(P>0.05),the proportion of NK cells in preeclampsia patients was significantly higher than that in normal pregnancy group(P<0.05).3.The expression of NK cell surface molecules CD158 B,TIGIT,LAG-3,NKG2 C,CD49A and CD69 decreased in the experimental group(P<0.05),increased CD158 A expression(P&L;0.05).4.The proportion of CD158 A + and CD158 B + in NK cells had predictive value for preeclampsia(P<0.05),the area under joint detection was higher than that under single detection.Conclusion: Statistical analysis showed that the proportion of NK cells increased and immune activity increased in patients with preeclampsia.The expression of NK cell surface molecules CD158 B,TIGIT,LAG-3,NKG2 C,CD49A and CD69 decreased,and the expression of CD158 A and CD158 B was significantly different.The combined detection was better than a single indicator in the prediction of preeclampsia.