Expression,Function And Clinical Significance Of OLA1 In Endometrial Cancer

Background and ObjectiveEndometrial carcinoma is a group of epithelial malignancies occurring in the endometrium and is one of the three major malignancies in women.In recent years,the incidence of endometrial cancer has increased and tended to be younger.The surgical effect of early endometrial cancer is good,and the survival rate of patients is high.However,the chemotherapy effect of late endometrial cancer is poor,and there is still a lack of effective treatment.Therefore,exploring the molecular mechanisms related to the proliferation,invasion and migration of endometrial cancer may be beneficial to provide new possibilities for the treatment of endometrial cancer.OLA1 belongs to the GTPase family of OBG and is widely expressed in a variety of species,including a variety of tumor and normal cell lines.OLA1 has been reported to be highly expressed in a variety of tumors,but its function in endometrial cancer and its regulation of related signaling pathways have not been reported.Therefore,exploring the functional mechanism and clinical significance of OLA1 in endometrial cancer may provide new ideas and methods for the diagnosis and treatment of endometrial cancer.Contents and Results1.The expression of OLA1 was higher in endometrial carcinoma tissues compared with the para-cancerous tissues;By analyzing the mRNA expression data set of endometrial cancer samples and tissue microarray data from the TCGA database,we found that OLA1 expression was higher in endometrial cancer than in para-carcinoma tissues.2.High expression of OLA1 indicates poor prognosis in endometrial cancer samples.We extracted the endometrial cancer clinical information data set from the TCGA database and combined the OLA1mRNA expression data with it for further analysis.Kruskal-Wallis test and logistic regression analysis showed that the expression level of OLA1 was closely related to clinicopathological factors in endometrial cancer.Kaplan-Meier method was used to calculate the overall survival rate of endometrial cancer patients with TCGA database data and tissue microarray data samples.Univariate and multivariate Cox analysis was used to analyze the relationship between the clinical factors and the expression level of OLA1 and prognosis.The results showed that the expression level of OLA1 was closely related to prognosis,and the high expression of OLA1 predicts poor prognosis in endometrial cancer patients.3.OLA1 promoted the proliferation,invasion and migration of endometrial carcinoma.CCK8,EDU,Transwell,Boyden and scratch experiments were performed to verify the effects of OLA1 on the proliferation,invasion and migration of endometrial cancer cells.The results showed that the high expression of OLA1 promoted the proliferation,invasion and migration of endometrial cancer cells.4.OLA1 may be involved in the WNT signaling pathway.GSEA enrichment analysis and Western Blot were used to preliminarily explore the possible signaling pathways involved in OLA1,and the results showed that OLA1 may be involved in the WNT signaling pathway.Conclusion1.The expression levels of OLA1 in endometrial carcinoma tissues were upregulated compared with that in para-carcinoma tissues.2.High expression of OLA1 indicates poor prognosis in endometrial cancer samples.3.OLA1 may promote the proliferation,invasion and migration of endometrial cancer cells via WNT signaling pathway.