Study On The Effect And Mechanism Of JIB-04 On The Proliferation And Apoptosis Of Cervical Cancer Cells

Objective: This article discusses the effect of JIB-04 on the proliferation and apoptosis of cervical cancer cells and its mechanism of action,providing a scientific basis for the development of small molecule inhibitors.Methods: HeLa and SiHa cells were selected as experimental objects.In routine culture,cervical cancer HeLa and SiHa cells were treated with different concentrations of JIB-04(diluted in DMSO).Use MTT and colony experiments to detect the inhibition rate and proliferation of cervical cancer cells by JIB-04;explore the effect and mechanism of JIB-04 on cervical cancer cell proliferation and apoptosis through Western blot experiment and q RT-PCR technology,and The effect of JIB-04 on histone demethylase LSD1.Results: The results of MTT method and cell colony formation experiments show that JIB-04 can inhibit the proliferation of cervical cancer cells.Western blot and q RT-PCR results showed that compared with the DMSO group,the expressions of cyclin Cyclin D1 and CDK4 in the JIB-04 group were significantly reduced;apoptosis-related proteins P53,caspase-3,Bcl-2,Puma The expression of LSD1 was significantly reduced;the expression of LSD1 was significantly reduced.Conclusion: 1.JIB-04 can regulate the expression of cyclin and inhibit the proliferation of cervical cancer cells.2.JIB-04 can reduce the expression of apoptotic proteins by regulating the activity of P53 and caspase3,thereby inhibiting the apoptosis of cervical cancer cells.3.JIB-04 can inhibit the activity of LSD1.