| Objective : To investigate the effects of moxibustion at Guanyuan and Mingmen points on the different expression of advanced glycation end products(AGEs),Receptors for advanced glycation end products(RAGE)and the gene of advanced glycation end products,interleukin-1(IL-1),TNF-alpha in brain and the different expression of IL-1,TNF-alpha in serum about the rats with subacute aging induced by D-galactose.In order to provide experimental basis for moxibustion at Guanyuan and Mingmen points to delay senility.Methods: 60 healthy male SD rats were randomly divided into blank group(10 rats)and aging model group(50 rats).The blank group was intraperitoneally injected with normal saline,while the model group was intraperitoneally injected with D-galactose 500 mg/kg-1 once a day for 60 consecutive days.Forty rats were randomly divided into model control group,Guanyuan acupoint treatment group,Mingmen acupoint treatment group and Guanyuan + Mingmen acupoint treatment group,with 10 rats in each group.The blank group and model control group were fed normally.But the treatment groups were treated with moxibustion for 4 weeks.After the treatment,the water maze experiment began.The water maze experiment lasted for 6 days.Platform exploration lasted for 5 days and space exploration lasted for 1 day.On the second day after the experiment,blood was collected from abdominal main vein of five groups of rats after anesthesia.After the blood was collected,the rats were executed.Brain tissues were collected quickly on the ice table and added with normal saline.Brain homogenate was made by homogenizer.The expression of AGEs,RAGE,IL-1 and TNF-alpha in brain homogenate and the expression of IL-1 and TNF-alpha in serum were determined by enzyme-linked immunosorbent assay,and the expression of RAGE m RNA in brain homogenate was detected by RT-PCR.Result:(1)Compared with the blank group,the latency of the model control group rats to find the platform was prolonged,and the number of times to cross the platform was reduced;there was a significant difference between the two groups(P<0.01).Compared with the model control group,the latent period of finding the platform in the three moxibustion treatment groups was shorter and the number of times of crossing the platform was increased(P<0.01 or P<0.05),but there was no significant difference among the three moxibustion treatment groups(P>0.05).(2)The expression of AGEs,RAGE,IL-1 and TNF-alpha in rat brain tissue was measured by ELISA.Compared with the blank group,the expression level of AGEs,RAGE,IL-1 and TNF-alpha in rat brain tissue in the model control group was significantly higher than that in the blank group(P<0.01).Compared with the model control group,the content of AGEs,RAGE,IL-1 and TNF-alpha in rat brain tissue in the three moxibustion treatment groups was significantly lower than that in The difference was statistically significant(P<0.01).The expression of IL-1 and TNF-alpha in rat serum: Compared with the blank group,the expression levels of IL-1 and TNF-alpha in rat serum of the model control group increased significantly,with significant difference(P<0.01);compared with the model control group,the contents of IL-1 and TNF-alpha in rat serum of the three moxibustion treatment groups decreased significantly(P<0.01).(3)The detection of RAGE gene expression in rat brain homogenate by RT-PCR: Compared with the blank group,the expression level of RAGE gene in the model control group was significantly higher than that in the model control group(P<0.01);compared with the model control group,the expression of RAGE gene in the three moxibustion treatment groups was significantly lower than that in the model control group(P<0.01).(4)Compared with the three moxibustion treatment groups,there was no significant difference in all the above indicators(P>0.05).Conclusion:(1)Moxibustion of Guanyuan and Mingmen points can improve the learning and memory of aging rats with D-galactose.(2)The mechanism of aging induced by D-galactose in rats may be related to the large amount of AGEs produced by D-galactose in vivo.(3)Moxibustion of Guanyuan and Mingmen points can decrease the expression levels of AGEs and RAGE.While the mechanism of delaying aging may be that the down-regulation of AGEs reduces the direct modification of biological macromolecules and the binding level with its specific receptor RAGE,They may be Inhibiting the signal transduction pathway of inflammation switch NF-KB can reduce the expression of pro-inflammatory factors IL-1 and TNF-alpha. |
PDF Full Text Request